不同临界不育温度温敏核不育水稻杂交f1代育性及ubl40基因表达研究

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1、AbstractRiceisastaplefoodformostofthepopulationintheworld，whoseyieldgrowthwillbeattributedprimarilytotheutilizationofitshybirdvigor．ThereexisttwomainsystemsinChina：thetraditionalthree-linesystemandthenoveltwo-linesystem．However，everyPTGMSlinemustbearalowerc

2、riticalsteirlitytemperaturepoint(CSTP)，whichisregardedasthelowermosttemperatureforPTGMSlinestokeeptheabsolutemalesterilitystatusunderlong-dayphotoperiod．Therefore，thelowermosttemperatureforPTGMSlineshasbecomeanimportantissuefortwo·linehybridbreeding．AnnongS

3、一1isthefirstIndicaTGMSinchina，butitiseasilyaffectedbythetemperaturebecauseofitshighcriticaltemperature．ZhulShaspromotedtherapiddevelopmentoftwo-linehybridriceduetoitslowercriticaltemperature．Therehavebeensomereportsaboutthechangerules．ofthelowermosttemperat

4、ureforPTGMSlines，whereas，therelationshipbetweentheregulationoftheTGMSline’Smolecularmechanismsanditsdifferentcriticaltemperatureremainstobefurtherstudied．ToguidebreedingTGMSLinewithlowercriticalsterilitytemperature，andtoprovideaclearreferencefortheinheritan

5、ceofTGMSline，theresearchwilltreatthepollenfertilityofthefilialgeneration11I万方数据whichoriginatesfromdifferentthermo—sensitivegeniemalesterile．ThelevelsofUbL40mRNAinmeiophaseundervarioustemperaturewillbestudied，too．Themainresultsareasfollows：1．AllTGMSlinesinri

6、ceweretreatedbylowtemperaturewaterof23．5"Cfor6dayswithintheirthermo-sensitivestagesoffertilityaltemation．Theresultsshowedthat：TheF1hybridweresterilewhichoriginatesfromZhulSwhohasthelowestcriticalsteirlitytemperaturepoint(CSTP)and35SwhohaslowerCSTP，ZhulSandt

7、heTGMSlineswhohavehigherCSTP(includingZhunS，AnxiangS)，ZhulSandAnnongS-1whohashigherCSTP,respectively．TheF1hybridhasalittlefertilewithstainablepollenrateof1％whichoriginatesfromparentmaterialswithlowercriticaltemperature(includingH628Sand35S)andZhunlSwhohashi

8、gherCSTP．TheF1hybridfertilewithstainablepollenrateof10％whichoriginatesfromparentmaterialswithlowercriticalsteirlitytemperaturepomtandthehighestcriticalsteirlitytemperaturepomt．TheF1hybridfertilewithsta