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《功能基因的研究流程(The general research process of functional genes)》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、ThegeneralresearchprocessoffunctionalgenesAbstact:Withthedevelopmentoftheresearchareasoflifesciences,moreandmorenewgenesandnewfunctionsofgeneswerediscovered.Itwouldbethemaintasktostudythefuctionofthesegenesinthenextfewyears.thisreviewintroducesseveralnewmethodsandprocedureson
2、thegenefunctionresearch.Keywords:genefunction;bioinformation;lifesciences1.Genecloning:UsingTRIzolreagentextractedtotalRNAfromthesamples.Usingtheblastwebpages,designtheprimers.TheproductofPCRrecyclingbyagarosegelelectrophoresisandpurifiingbyagentbox,senttothecompanysequencing
3、.2.Bioinformaticsanalysis:ThedatasForbioinformaticsanalysismainlycomesfromDNAChiptechnologyandotherrelatedtechnologyofgeneexpression.Withtheanalysisandcomparisonoftheknowngenesorproteinsequences,candidategenessequencelengthcanbedetermined[1].Anditprovidestheunknownproteingene
4、productsignaltransductionpathway,cellstructuretypesandcellpositioningforecastinformationandsoon.Bioinformaticsanalysiscanprovidevaluablereferenceinformationforunderstandingcomplexsignalingpathwayandtherelationshipwithothergeneproduct[2,3].3.Differentialexpressionprofiling:Ind
5、ifferentstagesanddifferenttypesoftissuesandcellsofontogeny,geneexpressionsaredifferent[1].WeregarditastheTime-spacegeneexpression.Therefore,beforetheresearchofafunctiongene,weshouldanalysdifferentionalexpressionprofiling[2,3].Differentialexpressionprofilingmainlyincludesthege
6、neexpressionprofilingandtheproteinexpressionprofiling[3].ThecommonmethodtoanalyseintranscriptionlevelisfluorescentquantitativePCR,Northernblot,situhybridizationslice,whilethesubcellularlocalization,Westernblotandimmunohistochemistryinproteinlevels[3].4.Validationofgenefunctio
7、nAfterreasonableprojectionsforthefunctionofthegene,weneedtostudyandvalidationitthroughExperiments[3].Usuallytheresearchstrategyistoimportgenesintoacellorindividual,throughtheexpressconditionofgenesinthebodyandobservationthechangeofcellbiologybehaviororindividualphenotypictrai
8、ts,wecanidentifygenefunction.Themainmethodsisgene transfer,geneknock
