多种抗原对树突状细胞促成熟作用的研究

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1、多种抗原对树突状细胞促成熟作用的研究田发青1季德兰2李举亨1唐玫琴1李惠卿3黄映彩3成小慧1（1深圳市龙岗区人民医院血液内科广东深圳518172）（2深圳市龙岗区人民医院中央悦城社康广东深圳518172）（3深圳市龙岗区人民医院血细胞实验室广东深圳518172）【摘要】目的：研究多种抗原促进树突状细胞（dendriticcells,DCs）成熟作用。方法：外周血单核细胞培养为DC,分别负载卡介苗、肺炎球菌疫苗、麻疹疫苗、K562及RPMI8266冻溶抗原后与T淋巴细胞共培养，以单独加入TNF-a为对照组，再加入相同体积培养基作为空白对照，观察DC形态，流式细胞仪分析DC表型，CCK8法测定混合

2、淋巴细胞反应（MLR）。结果：各组均培养出具有典型形态的DC,其中疫苗各组细胞CD83表达较肿瘤细胞各组明显升高，MLR作用强，而肿瘤组抗原乂较对照组和空白对照组为高，同时各实验组CDla表达较对照组和空白对照组明显降低，P<0.05,但各实验组CDla表达未见明显差异，P>0.05o结论：卡介苗、肺炎球菌疫苗及麻疹疫苗等微生物抗原促进DC成熟能力较K562及RPMI8266等肿瘤抗原能力强。【关键词】抗原；树突状细胞；CD83；混合淋巴细胞反应【中图分类号】R331.1+44【文献标识码】A【文章编号】2095-1752（2016）08-0385-02Multiplekindso

3、fantigeninducedendriticcellstomaturationITianFaqing,2JiDelan1LIJuheng,ITangMeiqin,3LIHuiqing,3HuangYingcai,lChengXiaohuiIDepartmentofHematology,LonggangDistrictPeople’sHospitalofShenzhen;2BloodCellLaboratory,LonggangDistrictPeople’sHospitalofShenzhen,Guangdong,518172,China【Abstract]Objec

4、tiveToinvestigatethecapabilityofmultiplekindsofantigens(Ags)topromotedendriticcells(DCs)mature.MethodsDCswereinducedfromperipheralbloodmononuclearcellsloadeddifferentAgssuchasBCG,pneumococcalvaccine，measlesvaccine，K562andRPMI8266-freeze-thawAgs,thentobeco-culturedwithTlymphocytes.TNF-aonlywasaddedas

5、controlgroup,andTNF-aandthesamevolumeofmediumasAgwereaddedasablankcontrol.DCmorphologywasobservedbyinvertedmicroscope,DCphenotypewasanalyzedbyflowcytometry,andmixedlymphocytereaction(MLR)wasdeterminedbyCCK8kits.ResultsCellsineachgroupwereinducedtohavethetypicalmorphologyofDC,whichCD83expressionwassi

6、gnificantlyhigherinvaccinegroupsthanintumorcellgroups,andwashigherintumorcellgroupsthanincontrolgroupandblankcontrolgroup,whileCDlaexpressionwassign讦icantlylowerineachexperimentalgroupthanincontrolgroupandblankcontrolgroup,P<0.05,butCDlaexpressionineachexperimentalgrouphadstillnosignificantdiffer

7、enee,P>O.O5.ConclusionMicrobialAgssuchasBCG,pneumococcalvaccineandmeaslesmaybetterpromoteDCmaturationthantumorcellantigenssuchasK562andRPMI8266・[Keywords]Antigen;Dendriticcell;CD83;Mixedlymphocyter