医学毕业论文人急性单核细胞白血病细胞系shi1在裸鼠体内的高致瘤性及其机制的初步研究

《医学毕业论文人急性单核细胞白血病细胞系shi1在裸鼠体内的高致瘤性及其机制的初步研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、人急性单核细胞白血病细胞系SHI1在裸鼠体内的高致瘤性及其机制的初步研宄姓名：2014年6月25日人急性单核细胞白血病细胞系SHI1在裸鼠体内的高致瘤性及其机制的初步研究作者：陈苏宁，薛永权，潘金兰，吴亚芳，王勇，岑建农【摘要】本研究确定人急性单核细胞白血病细胞系SHI1在裸鼠体内的高致瘤性，并对其成瘤机制进行初步探讨。将SHI1细胞接种至裸鼠皮下，观察肿瘤生长情况；取肿瘤组织行病理检测、R显带核型分析；RTPCR检测MLLAF6融合基因和VEGF基因的转荣；明胶酶谱法检测培养上清中基质金属蛋闩酶9(MMP9)和MMP2的表达；体外穿膜实验观察迁移能力。结果表明：注射SHI1细胞的16只

2、裸小鼠均于皮下出现肿块；瘤体由白血病细胞组成；注射的裸鼠中有MLL/AF6融合基因和VEGF基因的转录；在无血清培养上清中MMP9和MMP2的表达明显高于对照细胞；SHI1细胞有较强的迁移能力，MMP2的阻断抗体可显著抑制其体外迁移能力。结论：SHI1在裸鼠体内有极高的成瘤率，其机制可能与P53基因的异常、高水平VEGF基因的转录、金属蛋白酶的高表达和较强的体内浸润能力有关。【关键词】急性单核细胞白血病HighTumorigenicityofHumanAcuteMonocyticLeukemicCellLineSHI1inNudeMiceandItsMechanismAbstractTh

3、isstudywaspurposedtoexplorethetumorigenicityofanovelhumanmonocyticleukemiccelllineSHI1innudemiceanditsmechinism.ThetumorigenicityinmicewasevaluatedinsixteennudemicesubcutaneouslyinjectedwiththeSHI1cellline.Thetumorspecimenwasstudiedbytheconventionalpathologicexamination.Themononuclearcells(MNC)of

4、thetumorwasassayedbyRHGbanding,thetranscriptionofMLLAF6fusiongeneandtheVEGFgenewasdetectedbyRTPCR.GelatinzymographymethodwasusedtostudytheexpressionofMMP9andMMP2inthesupernatantoftheSHI1cellline.MatrigelinvasionassaywasemployedforthestudyofmigrationoftheSHI1cellinvitro.Theresultsshowedthatthetumo

5、rmasseswerefoundinallsixteenmudemiceaftersubcutaneousinjectionofSHI1cells，thetumormasswasmainlycomposedofleukemiacells，thetranscriptionofMLLAF6fusiongeneandVEGFgenewasprovedbyRTPCRanalysis，theexpressionsofMMP2andMMP9intheserumfreeculturesupernatantoftheSHI1celllineweresignificantlyhigherthanthose

6、inU937,K562,andNB4celllines.TheSHI1celllineexhibitedsignificantlyhigherinvitroinvasivenessthanotherleukemiacelllines，theblockingantibodyofMMP2couldinhibitthemigrationoftheSHI1celllinesignificantly.ItisconcludedthattheSHI1celllinepresentshighertumorigenicityinnudemicethanotherleukemiacelllineandth

7、emechanismisassociatedwithp53genealteration,hightranscriptionlevelofVEGFgene，highexpressionlevelofMMP，andsignificantlyhigherinvasiveness.Keywordsacutemonocyticleukemia;SHI1cellline;t(6;ll)(q27;q23);tumorigenicity;p53ge