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1、纳秒脉冲电场消融乳腺癌的实验研究［摘要］目的探索纳秒脉冲对乳腺癌高转移细胞株MDA-MB-231的治疗效果及其分子机制。方法纳秒脉冲电消融乳腺癌细胞及荷瘤小鼠后，检测接受纳秒脉冲治疗（实验组）及未接受纳秒脉冲治疗（对照组）的小鼠肿瘤细胞增殖、周期和凋亡相关指标，观察肿瘤的体积、生长形态及速度等的变化。结果纳秒脉冲在体外可以显著降低细胞活性，诱导细胞凋亡，表现为实验组CCK-8细胞活性抑制率与对照组比较，差异冇高度统计学意义（P〈0.01）,且各实验组间差异均有高度统计学意义（P<0.01）；投射电镜和线粒体电势实验观察到细胞出现凋亡，抑制肿瘤生长。结论纳秒脉冲可以通过诱导乳腺癌
2、细胞MDA-MB-231的凋亡而对肿瘤进行消融。［关键词］乳腺癌；MDA-MB-231；纳秒脉冲;消融［中图分类号］R730.5［文献标识码］A［文章编号］1673-7210（2015）06（a）-0004-06ExperimentstudyofnanosecondpulsedelectricfieldsablatebreastcancerXIEGuilinlCHENLuyan2CHENXinhua2WENHao3CHENXinmei41.DepartmentofGeneralSurgery,theSecondHospitalofShaoxing,ZhejiangProvinc
3、e,Shaoxing312000,China；2.DepartmentofGeneralSurgery,theFirstAffiliatedHospitalofZhejiangUniversity,ZhejiangProvince,Hangzhou310000,China；1.DepartmentofGeneralSurgery,theFirstAffiliatedHospitalofXinjiangMedicalUniversity,XinjiangUygurAutonomousRegion,Urumqi830054,China；4.SchoolofPharmacy,Shan
4、dongUniversityofTraditionalChineseMedicine,ShandongProvince,Ji'nan250355,China[Abstract]ObjectiveToinvestigatethetreatmenteffectofnanosecondpulsedelectricfields(nsPEFs)onhigh1y-metastatichumanbreastcamcercelllinoMDA-MB-231invitroandinvivotheandthepossiblemolecularmechanism.MethodsAftertreatm
5、entofnsPEFsindifferentparameters,theapoptosis,proliferationandinvasionofMDA-MB-231cellsinratsofnanosecondpulsetherapy(experimentalgroup)andnon—nonosecondpulsetherapy(controlgroup)wereexaminedwithCCK-8,TUNEL,f1owcytometer,transmissionelectronmicroscopy(TEM).Thevolumoftumorsize,grothpatternand
6、speedwereobserved・ResultsInvitro,nsPEFsinhibitedthecellviabilityandinclucedcellapoptosis.ThecellviabilityofCCK-8intheexperimentalgroupwasreducedsignificantlycomparedwithcontrolgroup,thedifferencewasstatisticallysignificant(P<0.01)，andthedifferencesamongdifferentexperimentalgroupwerestatistic
7、allysignificant(P<0.01)；italsopresentedmitochondriadegeneration.FlowcytometershowedthatnsPEFscouldinhibitcancercellproliferation.ConclusionnsPEFscanablatehumanbreastcancerMDA-MB-231byactivatingcaspasesignalingpathway.［Keywords］Breastcancer；MDA-MB-2
