忍冬枝枯病病原菌地研究

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1、...编号：河南中医学院２０１２届硕士研究生学位论文忍冬枝枯病病原菌研究研究生姓名：毕淑娟导师：刘红彦研究员指导组成员：刘玉霞副研究员陈随清教授董诚明教授学科、专业：生药学所属院、部：药学院中国﹒郑州２０１２年４月１０日............硕士学位论文原创性声明本人所呈交的硕士学位论文，是在导师刘红彦研究员的指导下，独立进行科学研究工作所取得的成果。除文中已特别加以注明引用的内容外，论文中不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出重要贡献的个人和集体，均已在文中以明确方式标明并致谢。本人完全意识到本声

2、明的法律结果由本人承担。特此声明。论文作者（签名）：年月日硕士学位论文使用授权声明本人已完全了解河南中医学院有关保留、使用硕士学位论文的相关规定，同意学校保留或向国家有关部门、机构送交本学位论文的复印件和电子版，允许论文被查阅或借阅。本人授权河南中医学院可以将本论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或其他复制手段保存、汇编本论文（注：保密论文在解密后适用本授权声明）。特此声明。论文作者（签名）：导师（签名）：年月日年月日............目录摘要····························

3、························································1Abstract················································································3前言···················································································6实验研究································

4、··············································7第一部分忍冬枝枯现象调查及病原菌分离鉴定·······························71仪器与材料·······································································71.1实验仪器·····································································71.2实验材料·······

5、······························································72实验内容与方法·································································72.1忍冬枝枯现象田间调查·······················································72.2忍冬枝枯病疑似病原菌的分离··············································

6、·72.3忍冬枝枯病疑似病原菌的致病性测定·······································82.4忍冬枝枯病原菌培养性状观察···············································82.5rDNA-ITS序列及翻译延伸因子TEF-1α基因部分序列鉴定··············83实验结果与分析································································11第二部分四株镰刀菌的生物学特性考察···

7、··································241仪器与材料······································································241.1实验仪器····································································241.2实验材料····································································24

8、2实验内容与方法································································242.1温度对镰刀菌菌丝生长及产孢的影响·······························