诱导膜在骨修复中的作用机制

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1、TheMechanismofActionofInducedMembranesinBoneRepair.诱导膜在骨修复中的作用机制AbstractBACKGROUND:Inducementofforeign-bodygranulationtissueisarelativelynoveltherapeuticmodalityinbonerepair.Atwo-stagebonereconstructionmethod,knownastheMasquelettechnique,combinesinducementofagranulationtissuemem

2、braneandsubsequentboneautograftingasabiphasictechniqueallowingreconstructionoflargebonedefects.Inlightoftheiralreadywell-characterizedosteogenesis-improvingcapabilitiesinanimals,weperformedthistranslationalstudytoinvestigatethesemembranesinpatients.摘要背景：诱导异物肉芽组织在骨修复中是一种较新的治疗方法。两

3、阶段骨重建方法，即熟知的Masquelet技术，是结合诱导肉芽组织膜和随后的骨自体移植的双向技术以允许大段骨缺损重建。根据它们在动物实验中良好的提高成骨能力特点，我们将这些膜植入患者中进行研究。METHODS:Fourteenpatientswithcomplicatedfracturesandbonedefectswererandomlyselectedforthisstudy.Biopsysamplesofforeign-body-inducedmembraneswerecollectedatdifferenttimepointsduringsch

4、eduledsurgicalprocedures.Themembraneswereco-culturedwithmesenchymalstromalcells,anddifferentiationintotheosteoblasticlineagewasassessedbymeasuringalkalinephosphataseactivity,aminoterminalpropeptideoftype-Iprocollagen(PINP)production,andCa2+concentration.Histologicalcharacteristi

5、cswereevaluatedwithimageanalysis.Quantitativereversetranscriptionpolymerasechainreactionwasusedtomeasurevascularendothelialgrowthfactor(VEGF),interleukin-6(IL-6),andtype-Icollagen(Col-1)expression.方法：本研究将14例复杂骨折并骨缺损的患者随机分组。按预定的手术方法在不同时间点活检收集异物诱导的膜。这些膜和间充质干细胞共同培养，通过测定碱性磷酸酶活性、I型胶原

6、(PINP)产物氨基端前肽、以及钙离子浓度评估其分化成成骨细胞谱系。用图像分析对组织学特点进行评价。应用定量逆转录聚合酶链反应法对血管内皮生子因子(VEGF)、白细胞介素-6(IL-6),以及I型胶原蛋白(Col-1俵达进行测量＜RESULTS:Theinducedmembraneswerecharacterizedhistologicallybymaturatingvascularizedfibroustissue.Thevascularizationwasgreatestinone-month-oldsamplesanddecreasedto<60

7、%inthree-month-oldsamples.One-month-oldmembranesampleshadthehighestexpressionofVEGFrIL-6,andCol-1,whereastwo-month-oldmembranesexpressed<40%ofthelevelsoftheone-month-oldmembranes.Specificalkalinephosphataseactivity,PINPproduction,andCa2+concentrationwereincreasedinco-cultureswhe

8、namembranesamplewaspresent.Inculturesofone-mont