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1、基金项目:广西科技厅科技基础条件平台建设项目科技条件专项(10-046-04)作者简介:黄兴振,讲师,硕士,研究方向:新药开发研制,E-mail:huangxingzhen6@yahoo.com.cn,TEL:0771-5358272，13507718806*通讯作者:谭珍媛，助理研究员,硕士,研究方向:新药新剂型的研究与开发,E-mail:yuan6www@163.com,TEL:0771-5358272，13桂产山银花栽培品种的鉴定与含量测定黄兴振1，王志萍2，谭珍媛1*(1广西医科大学药学院，南宁，530021；2.广西中医药大
2、学药学院，南宁市530001)摘要：目的对桂产山银花栽培品种进行品种鉴定和质量评价，为广西忍冬属药用植物进一步开发和利用提供依据。方法对巴马和忻城产山银花进行性状鉴定；采用显微鉴别观察其粉末的不同点；采用薄层色谱法对其主要功效成分绿原酸进行了鉴别；采用高效液相色谱法测定其中绿原酸含量。结果鉴定忻城山银花为红腺忍冬，巴马山银花为华南忍冬，两地产山银花的绿原酸含量分别为27.2581和15.9758mg·g-1。结论建立的方法可靠，为桂产山银花种质提供了客观综合的评价，也为筛选优质高产山银花提供了理论依据。关键词：山银花；鉴定；质量评价；
3、绿原酸；含量测定中图分类号：R284.1；R917.101文献标志码：A文章编号：IdentificationanddeterminationofLoniceraeconfusecultivatedvarietiesinGuangxiSongFang-Ming1,WangZhi-Ping2,TanZhen-Yuan1*(1.SchoolofPharmaceuticalScience，GuanXiMedicalUniversity,NanNing,China;2.SchoolofPharmaceuticalScience,GuangXi
4、UniversityofChineseMedicineNanNing,China;)Abstract:ObjectiveToidentifyLoniceraeconfuseresourcesandtoprovidethequalityevaluationofthoseforpromotingtherationaldevelopmentandutilizationofLoniceraLinn.inGuangxi.MethodsBasedonthetraitsofbudsinBamaandXincheng,thecharacteriden
5、tificationandmicroscopicalcharacteristicswereusedtomakeclearthedifferencesoftwokindsof.Loniceraeconfuse.Chlorogenicacid,themainefficacycomponentinLoniceraeconfuse,wasidentifiedthroughTLCanalysis.ThecontentofchlorogenicacidinLoniceraeconfusefromdifferentareaswasdetermine
6、dbyHPLC.ResultLoniceraeconfusefromxinchengwasLonicerahypoglaucaMiq.LoniceraeconfusefrombamawasLoniceraconfuseDC.ThereweredifferencebetweenLoniceraeconfusefromdifferentareasinmacroscopicandmicroscopiccharacteristics.Thechlorogenicaciddisplaybluespotobviously.Thecontentof
7、chlorogenicacidinxinchengandbamawas27.2581mg·g-1and15.9758mg·g-1respectively.ConclusionThemethodisfeasible,whichcanProvideobjectiveandcomprehensiveevaluation,alsocanprovidedacademicbasisforscreeninghighyieldandqualityofLoniceraconfusainGuangxi.Keywords:Loniceraeconfuse;
8、Identification;qualityevaluation,chlorogenicacid;determination山银花是忍冬科忍冬属植物红腺忍冬LonicerahypoglaucaMiq..，灰毡毛忍冬Lon
