s期激酶相关蛋白2在宫颈上皮内肿瘤和宫颈鳞状细胞癌中的表达及意义论文

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1、S期激酶相关蛋白2在宫颈上皮内肿瘤和宫颈鳞状细胞癌中的表达及意义论文【摘要】目的探讨S期激酶相关蛋白2（Skp2）在正常宫颈上皮、宫颈上皮内肿瘤(CIN)、宫颈鳞状细胞癌(SCC)中的表达及其在宫颈癌演进过程中作用和意义。方法用鼠抗人Skp2单克隆抗体，免疫组化两步法标记12例正常宫颈组织、30例宫颈上皮内肿瘤、30例宫颈鳞状细胞癌组织。结果正常宫颈组织、宫颈上皮内肿瘤和宫颈鳞状细胞癌Skp2表达阳性率分别为0%、76.6%、83.3%。正常宫颈组织与宫颈上皮内肿瘤、正常宫颈组织与宫颈癌Skp2的表达有显著差

2、异(P＜0.01)。而宫颈上皮内肿瘤与宫颈癌比较Skp2的表达无显著性差异(P＞0.05)。结论Skp2参与了正常宫颈组织向宫颈上皮内肿瘤的演变,可能与宫颈鳞状细胞癌的发生和发展有关。【关键词】S期激酶相关蛋白2；宫颈上皮内肿瘤；宫颈鳞状细胞癌【Abstract】ObjectiveToinvestigatetheexpressionofSphasekinaseassociatedproteint2Skp2anditssignificanceincervicalintraepithelialneoplasia

3、andsquamouscellcarcinoma.MethodsTheimmunohistochemiscalstaininginetheexpressionlevelofSkp2insamplesof12casesofnormalcervix,30casesofcervicalintraepithelialneoplasia,and30casesofcervcalsquamouscellcarcinoma.ResultsTheSkp2expressionrateinnormalcervix,cervical

4、intraepithelialneoplasiaandcervcalsquamouscellcarcinomaalcervix(P＜0.01).Buttherea.ConclusionTheincreasingSkp2expressioninvolvesinthedevelopmentandprogressionfromnormalcervixtocervicalintraepithelialneoplasia,andmaybecorrelatedtotheonsetandprogressofcervical

5、carcinoma.【KeyS,AdamsonA，linZ,etal.ExpressionofSkp2,ap27(kip1)ubiquitinligase,inmalignantlymphoma:correlationpliconthatisfrequentlydetectedinsmallcelllungcancers［J］.AmJPathol,2002,161(1):207216.［7］MasudaTA,InoueH,SonodaH,etal.Clinicalandbiologicalsignifica

6、nceofSphasekinaseassociatedprotein2(Skp2)geneexpressioningastriccarcinoma:modulationofmalignantphenotypebySkp2overexpression,possiblyviap27proteolysis［J］.CancerRes,2002,62(13):38193825.［8］YokoiS,YasuiK,SaitoOharaF,etal.Anoveltargetgene,Skp2,pliconthatisfr

7、equentlydetectedinsmallcelllungcancers［J］.AmJPathol,2002,161(1):207216.［9］ChiarleR,FanY,PivaR,etal.Sphasekinase-associatedprotein2expressionanddefinescellinSphase［J］.AmJPathol,2002,160(4):14571466.［10］GstaigerM,JordanR,LimM,etal.Skp2isoncogenicandoverexp

8、ressedinhumancancers［J］.ProcNatlAcadSciUSA,2001,98(9):50435048.［11］Doaofthecervixdoesnotcorrelateinverselywithp27expression［J］.IntJCancer,2003,105(3):326330.