补肾抗衰汤对氧化应激所致的秀丽隐杆线虫生育缺陷的影响论文

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1、补肾抗衰汤对氧化应激所致的秀丽隐杆线虫生育缺陷的影响论文曹新国,侯莉莉,陈军霞,陆勤【摘要】目的：探讨补肾抗衰汤对秀丽隐杆线虫因氧化应激诱导所致生育缺陷的作用。方法：将终浓度为0.33g/mL的补肾抗衰汤按25%、50%、75%和100%梯度稀释后对L4期线虫培养。应激方式采用紫外线照射［20J/(m2·min)］、热休克（36℃,2h）和百草枯(2mmol/L,2h)处理。通过子宫内卵数、卵的大小和传代时间来评价秀丽隐杆线虫的生育能力。结果：野生型N2线虫经过紫外线照射、热休克和百草枯处理后，子宫内卵数、卵的大小均显著减少.freelev1突变体线虫受损的生育能力。结论：氧化应激对

2、生育能力有负面影响，高浓度补肾抗衰汤可明显逆转氧化应激所致的线虫生育缺陷。【关键词】补肾抗衰汤;氧化应激;生育;线虫Thereproductivesuccessesofanimalsandhumansdependonaseriesofcarefulneuroendocrineevents,andthereproductionfailureingforanyoneoftheseeventsisdisrupted［1］.Undersuchconditions,animalreproductionappearstobethemostvulnerable［1］.Forexample,ent,a

3、ndimpairedembryonicdevelopment［2］.Stresscanbedefinedasanevent(physical,.freelental,psychological,etc.)eostasisoftheanimals.Inmammals,stressimpactsonthereproductiveaxisatthehypothalamusandthepituitarygland［3］.Variousstressfulconditionshaveadverseeffectsonreproductionofanimals,andthestressconditio

4、nsincludeenvironmentalfactors(i.e.,temperature,humidity,metalsandirradiation),physicalfactors(i.e.,transport,shearing,restraintandstrenuousexercise),metabolicfactors(i.e.,insulininducedhypoglycaemia),immunologicalfactors(i.e.,infectionandendotoxinsadministration),andpsychologicalfactors(i.e.,is

5、olation,socialinteractionsandmentalarithmetictasks)［15］.Caenorhabditiselegans,aclassicalmodelorganism,isafreelivingnematode,specializedmuscles,anddigestiveandreproductivesystems［6］.ThereproductiveprocesshasbeeninvestigatedextensivelyinC.elegans［79］.C.eleganshermaphroditesareselffertile,andthe

6、irreproductiveprocessismodulatedbydiverseenvironmentalcues［79］.Amongtheseenvironmentalcues,environmentalstresscanaffectthereproductiveprocessofnematodessignificantly.ThedatafromCoohilletal［10］shoentandfertility.Thereproductiveprocesscanalsobealteredbyheatstressinnematodes［11］.Electromagicnanopu

7、lseexposureent［12］.Inaddition,exposuretoheavymetalsoflead,silver,nickelandzinceinexposednematodes［1316］.BushenKangshuaiTang(BKT),apoundtraditionalChineseherbalmedicine,hasbeenentofprematureovarianfailure(POF).Thismedicineca