dna固定在纳米颗粒表面的方法

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1、磷酸化的DNABiotin-DNA炔基修饰的DNA巯基修饰的DNA氨基修饰的DNA金银纳米颗粒碳纳米管、石墨烯二氧化硅纳米颗粒量子点上转换纳米颗粒磷酸化的DNA与TiO2π-π堆积，如单链DNA与石墨烯，碳纳米管静电吸附1、Biotin-DNA2、炔基修饰的DNA及其他生物分子3、NH2-DNA氨基羧基氨基羧基氨基氨基1）EDC和NHSForbestresultsintwo-stepreactions,performthefirstreactioninMESbuffer(orothernon-amine,non

2、-carboxylatebuffer)atpH5-6,thenraisethepHto7.2-7.5withphosphatebuffer(orothernon-aminebuffer)immediatelybeforereactiontotheamine-containingmolecule氨基羧基DCC（N,N′-Dicyclohexylcarbodiimide）氨基-氨基1）BS(PEG)nPEGCrosslinkers-AmineReactive2）TSAT(Tris-succinimidylamino

3、triacetate)WaterWaterNHSesters:pH7-93）DSS(disuccinimidylsuberate)4）BSOCOES(bis[2-(succinimidyloxycarbonyloxy)ethyl]sulfone)5）DSG(disuccinimidylglutarate)6）DTSSP(3,3´-Dithiobis[sulfosuccinimidylpropionate])WaterWaterWaterDMSOorDMF8）BS³(Bis[sulfosuccinimidyl]s

4、uberate)9）EGS[ethyleneglycolbis(succinimidylsuccinate)]10）Sulfo-EGS[ethyleneglycolbis(sulfosuccinimidylsuccinate)]7）DST(disuccinimidyltartrate)WaterWaterDMSOorDMFDMSOorDMF4、巯基化的DNA1Au-SAg-S2巯基氨基3巯基羧基4巯基羟基5巯基巯基巯基-氨基1）AMAS(N-[α-maleimidoacetoxy]succinimideeste

5、r)NHSesters:pH7-9；Maleimides:pH6.5-7.5；Usually7.2-7.5。TwostepsreactionDMSOorDMF2）BMPS(N-[β-maleimidopropyloxy]succinimideester)DMSOorDMF3）EMCS(N-[ε-maleimidocaproyloxy]succinimideester)DMSOorDMF4）Sulfo-EMCS(N-[ε-maleimidocaproyloxy]sulfosuccinimideester)Wate

6、r6）Sulfo-GMBS(N-[γ-maleimidobutyryloxy]sulfosuccinimideester)5）GMBS(N-[γ-maleimidobutyryloxy]succinimideester)DMSOorDMFWater7）BMPA(N-β-maleimidopropionicacid)EDC，0.1molMESpH4.7Maleimidesreactwithsulfhydryls(–SH)atpH6.5-7.58）LC-SMCC(Succinimidyl-4-[N-maleimid

7、omethyl]cyclohexane-1-carboxy-[6-amidocaproate])DMSOorDMF10）Sulfo-SMPB(sulfosuccinimidyl4-[p-maleimidophenyl]butyrate)9）SMPB(succinimidyl4-[p-maleimidophenyl]butyrate)DMSOorDMFWater11）Sulfo-LC-SPDP,LC-SPDP,SPDP巯基-羧基巯基-羟基异氰酸脂在水溶液中很容易水解，所以最好用有机溶剂如DMSO、DMF等。如在水

8、相中，则要使用pH8.5的碱性缓冲溶液，避免使用含羟基的缓冲液如tris缓冲液，且要注意含羟基的分子不能有氨基和巯基，因为pH＞7.5顺丁烯二酰亚胺失去对巯基的特异性，会同时与氨基和巯基发生反应。巯基-巯基pH6.5-7.5表征：1、定性（红外表征，如交联剂的特征峰；测颗粒的在260纳米处的紫外吸收，跑电泳，电位，粒径变化）2、定量（测颗粒或上清的紫外吸收，荧光，热重力分析）谢谢大家