oviductus ranae method of extraction and isolation of active

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1、OviductusRanaemethodofextractionandisolationofactive【Abstract】OviductusRanaeisoneofvaluabletraditionalchinesemedicine,edicinaleffects.Forfurtherresearchandapplication,thispaperreviealeRanatemporariachensinensisDavid,edicineacopoeiafromedition1985toedition2005,andithaseffectsofinvigoratingthespleenan

2、dthestomach,nourishingyinandthekidney,moisteningthelungandpromotingtheproductionofthebodyfluid[1].OviductusRanaeisavaluablechinesemedicine,nia,night-sacteric　　syndromeandsoon.OviductusRanaecontainsproteins,aminoacids,fattyacidsandesters,steroids,nucleicacids,sterols,amides,hydantions,vitaminsandmany

3、otheractiveingredients.ThispaperrevieentoftheextractionandisolationtechnologiesofefficientponentsinOviductusRanae,inordertofacilitatefurtherresearchanddevelopmentuse.　　1Fattyacidsandesters　　Theseconponentshavesimilareffectasprednisonestimulatingthesynthesesofprotein,thusimprovethepoing[3、4]gothexade

4、canoicacid,2,3-dihydroxypropylester,hexadecanoicacid,2-hydroxy-1,3-propanediylester,9-octadecenoicacid,2,3-dihydroxypropylester,octadecanoicacid,hexadecanoicacidethylesterandoctadecanoicacidethylesterfromOviductusRanaebysupercriticalcarbondioxidefluidextractiontechnologybiningcolumnchromatographieso

5、nsilicagel（mobilephase:petrolether-chloroform,petrolether-ethylacetate）,gelandAgNO3(mobilephase:chloroform-acetone).,SongSJ,,SongSJ,XuSX,etal.StudyonthechemicalconstituentsoftheOvumOilofRanatemporariachensinensisDavid[J].ChineseJournalofMedicinalChemistry,2001,11(4):224.　　[5]edicine,2006,28(1):93-94

6、.　　[6]edicalJournal,2003,16（2）:97.　　[9]ZhaoicalconstituentsofOviductusRanae[J].JournalofShenyangPharmaceuticalUniversity,1997,14(1):24.　　[10]edicinalMaterials,1996,19(2):90.　　[12]FanYL,CuiXS,YaoYX,etal.ResearchadvanceonOviductusRanaeposition[J].JournalofJilinAgriculturalUniversity,1996,18(3):105.　　[

7、13]ethod[D].Changchun:Jilinuniversity,2007.　　　　简介：王丽丽（1982-），女，吉林松原人，石家庄医学高等专科学校，助教，硕士学位，研究方向：天然药物有效成分的研究。　　*通讯：王永生（1962-），男，吉林长春人，吉林大学，博士，硕士生导师，研究方向：天然药物有效成分的研究。