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1、本科生毕业设计(论文)摘要决明子是一种常用中药,富含黄酮,蒽醌和多糖,具有广泛的药理活性。现代药理学研究又表明,决明子具有降血压、降血脂,保肝及抑菌等活性,被应用于多种降脂减肥的成方制剂中。同时,决明子又可作食用中药,是保健饮料的良好原料。实验过程中测定了决明子中黄酮类化合物的含量,采用乙醇回流水浴提取黄酮,对比研究提取时间、乙醇浓度、浸出温度和料液比等因素对提取效果的影响,再进行正交实验优化工艺条件。含量测定应用紫外—可见分光光度法进行测定,并使用NaNO2-Al(NO3)3-NaOH显色体系。实
2、验结果表明乙醇提取法效果最佳。在最佳提取条件75%乙醇为提取剂,80℃下提取,提取时间3.5h,料液比1:30时。以芦丁为标准物质,绘制的标准标准曲线其回归方程为C=96.838A-2.8675,相关系数R2=0.9951。又测得提取液吸光度为0.428,黄酮浓度为37.0μm/mL,提取率为88.61%。关键词:决明子,黄酮类化合物,乙醇,提取工艺IV本科生毕业设计(论文)AbstractSemenCassiaenatraditionalChineseherbalmedicine,whichisa
3、richsourceofflavones,polysaccharidesandanthraquinone,havingawidespreadactivityinbiology.Modernpharmacologystudyalsoshowsthat,cassiaseedalsohavefallbloodpressure,fallhematicfat,protectliverandbacteriostasisactivities,beappliedtovariouscholesterol-loweri
4、ngdietdrugsinpreparations.Meanwhile,cassiaseedandeatingChinesetraditionalmedicine,isthegoodhealthdrinkrawmaterial.Somechemicalseparationfactorswereadoptedtoextractflavonoids.Thisexperimentdeterminedthecontentofflavonoidsincassiaseed,andcassiaseedtheext
5、ractiontechnologyofflavonoidswerestudied.EthanolCondensingRefluxExtractionwereappliedtoextractflavonoids.Whenextracting,FiveextractionfactorsofflavonoidsfromSemenCassiaenwereinvestigatedandcompared,includingextractingtime,ethanolconcentration,extractin
6、gtemperatureandratioofliquidtosolid.Againtheoptimizationofprocessconditionsfororthogonalexperiment.QuantificationwasperformedbymeansofUV–VisspectrophotometrywithchromogenicsystemofNaNO2–Al(NO3)3–NaOH.Experimentsshowthatthattheethanolextractionwasbetter
7、thantheother.Thefoundoptimumextractionconditionswerethattheethanolconcentrationwas75%(v/v),solid–liquidratiowas1:30,extractingtemperaturewas80°C,extractingtimewas3.5h.Usingtheconcentrationofrutinstandardsolutionasabscissaandtheabsorbencyasy-coordinate,
8、thelinearchartwasconstructedandthestandardcurvetheregressionequationwasC=96.838A-2.8675(R2=0.9951).Andmeasurementextractspectrophotometryfor0.428andtheyieldofflavonoidswas88.61%.Keywords:cassiaseed,flavonoids,ethanol,cxtractiontechnolog