急性胰腺炎患者外周血microrna-216a测定及临床意义

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1、授予单位代码10089学号或申请号20153145中国图书分类号R576HebeiMedicalUniversity硕士学位论文专业学位急性胰腺炎患者外周血microRNA-216a测定及临床意义研究生：靳晓旭导师：冯志杰教授专业：内科学二级学院：河北医科大学第二医院2018年3月目录中文摘要···············································································1英文摘要·············································

2、··································3英文缩写···············································································5研究论文急性胰腺炎患者外周血microRNA-216a测定及临床意义前言···············································································6材料与方法····································

3、··································7结果···············································································12附图···············································································14附表·········································································

4、······19讨论···············································································21结论···············································································22参考文献·········································································23综述microRNA-216a在急性胰腺炎发病

5、中的作用···························25致谢·····················································································36个人简历···············································································37中文摘要急性胰腺炎患者外周血microRNA-216a测定及临床意义摘要目的：在通过测定AP患者外周血中测定microRNA-216a的水

6、平，并探讨其临床意义。方法：1.标本的选取：收集2017年8月-11月入住河北医科大学第二医院消化内科AP患者34例，其中MAP10例、MSAP6例、SAP18例。同时选取健康者31例。2.实验过程1)标本留取：入院24h内抽取AP组外周血3-5ml，进行4℃离心后即血浆待测样本，立即放入-80℃冰箱冷冻。2)测定方法：采用实时荧光定量PCR，分别在AP患者及健康人群中测定microRNA-216a水平。3.统计学分析：采用SPSS23.0进行统计学分析。计量资料用均数±标准差表示，并采用成组t检验，方差分析及person相关分析等，P<0.05

7、为差异有统计学意义。结果：1.AP组和健康人组外周血的microRNA-216a值分别为6.71和1.20，AP组明显高于对照组，具有统计学意义（P<0.0025）；2.对AP患者各型进行方差分析，SAP组患者microRNA-216a水平高于MAP、MSAP组（P<0.03），具有统计学意义；3.person相关分析：microRNA-216a的水平与APACHEII评分、Ranson评分、MCTSI评分存在正相关性，与淀粉酶无相关性；4.microRNA-216a鉴别AP患者的敏感性为94.1%，特异性为83.9%。结论：1.AP组外周血mi

8、croRNA-216a水平，明显高于健康人组。2.microRNA-216a水平与AP严重程度呈正相关性。microRNA-216a的水