傅里叶变换近红外漫反射光谱法结合化学计量学技术分析冻干粉针剂的方法学分析

《傅里叶变换近红外漫反射光谱法结合化学计量学技术分析冻干粉针剂的方法学分析》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、重庆医科大学硕士研究生学位论文withdiffusereflectanceFT．NIR．2．EstablishamethodtodetermineactivecomponentcontentsoflyophilizedpotassiumsodiumdehydroandroandrographolidesuccinateforinjectionwithdiffusereflectanceFT-NIR．3．Establishamethodtodiscriminatecakestructuresoflyophilizedpotassiumsodiumdehydro

2、androandrographolidesuccinateforinjectionwithdiffusereflectanceFT—NIR．M匝THoDS1．MeasurementofFouriertransforillnearinfrareddiffusereflectancespectra(NIRDRS)oflyophilizedpotassiumsodiumdehydroandroandrographolidesuccinateforinjectionScanregion：10000-4000cm。1；numberofscans：64；resoluti

3、on：8cm一1；temperature：22±lOC；humidity：55±5％RH．2．Determinationofthereferencevaluesoflyophilizedpotassiumsodiumdehydroandroandrographolidesuccinateforinjection(1)Determinationofmoisturecontents：thereferencevaluesofmoisturecontentswereobtainedbyKFT．(2)Determinationofthecontentsofactive

4、component：thereferencevaluesofthecontentsofactivecomponentweremeasuredbyHPLC．Column：C18column(250mmx4．6mm，5rtm)，mobilephase：methanol—potassiumdihydrogenphosphate(pH3．0；0．05mol／L)(64：36，v／v)，flowrate：1．0ml／min,columntemperature：400C，injectionvolume：重庆医科大学硬士研究生学位论文20ILl，detectionwave

5、length：251nm．(3)Discriminationofcakestructures：thecakestructureswerediscriminatedbyVI．3．Calibrationandvalidationofthemodelsfortheanalysisoflyophilizedpotassiumsodiumdehydroandroandrographolidesuccinateforlnjectlon(1)Calibrationandvalidationofthemoisturemodels：themoisturemodelsofNIR

6、Swereestablishedbyback．-．propagationartificialneuralnetwork(BP-ANN)andpartialleastsquares(PLS)．(2)Calibrationandvalidationoftheactivecomponentmodels：theactivecomponentmodelsofNIRSwereestablishedbyBP-ANNandPLS．(3)Calibrationandvalidationofthecakestructuremodels：thecakestructuremodel

7、sofNIRSwereestablishedbyself-organizingmap(SOM)anddiscriminantanalysis(DA)．RESULTSI．DeterminationofmoisturecontentsoflyophilizedpotassiumsodiumdehydroandroandrographolidesuccinateforinjectionwithdiffusereflectanceFT二NIR(1)BP．ANNmodel：thenetworkstructureparameterswere6-5—1，RMSECVwas

8、0．2376，RMSEPwasO．1471，Roft