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时间:2018-11-06
《玉米花粉多糖对猪瘟弱毒苗免疫效果的影响研究》由会员上传分享,免费在线阅读,更多相关内容在工程资料-天天文库。
1、广西大学硕士学位论文I*猪瘟弱毒苗免疫效果的影响研宄姓名:温纳相H青学位级别:硕士:传染病学与预防医階导教师:陆芹章2000.6.1摘要本研究采用醇沉水法提取玉米花粉3509,获得粗提复合物38.3反应、a萘酚反应、间苯二酚反应、碘化反应及高效液相色谱法鉴定,证多糖。/用上述多糖按低剂量(50mg)、中剂量(100mg)、高剂量(2猪瘟弱毒细胞冻干苗共同免疫4组非猪瘟免疫猪,每组3头,每隔7天5测定体液免疫和细胞免疫水平。其结果表明:(1)间接血凝试验测得各的IHA抗体效价均高于对照组,其差异显著(P2、%、2对照组(15.91%),其差异显著(P<0.OS);(3)T淋巴细胞转验组的T淋巴细胞转化率(55.32°%、5639%、54.27°%)均高于对照组及未加PHA组1.34%,其差异显著(p3、haStudiesoeffectnmmuneResponsetoAttenuatedHogcholeraveseCstrainAbstract:Onthisstudyweusedalcoholprecipitationtoextract!ve3509ofposen,andacquired38.89ofcrudeextracts.AfterusedcrudeexffJo(anthraone,mo1ish,se1iwanoff,ioJiJe)reactionacthigpressurliquidchroma1togrtestidentification,canheaphalidati4、onthapolysaccharidesExperimentalgroupswerevacwith①50ragpolySSCChaFidcinatedioOmgpsaccharides+HCLV;③200mgpolysacchm’ides+HCLV;④ph:o1yS01ineS01UtiOn+HCLV;(eVeygrouhavthreenon—immpigs)every'neuneabstraction,respectively;nj^gdetemincell-.immunityedantibodylevelofexperiigherestir5、ive-Erossetcmoralmenta]diffe»fexp)up15.91transfc32%,56.3oughtroupassCD8anta1gria1theistandard.Theresushowed①inRshancontrolgroup.Thougtlymphocyteactive—Erosett:Thehancontrolgrcgroupare:21.84°/25.46%21.92%allhigheest(P<0.#5)significanceofdefference;③Tlymphocytetransformationrateofexperimentalgr6、oupare55.herthancontrolgro47.00%,andnotaddPHAgroup1,34%Thrup口<0.05)signigieanceofdefference;④TiymphocysubgteexperimentalgroupsofavergevalueT1CD3、CIM>hamhatofcontrolgroups,buttheNK1cellofexperime01groupwerenosignificanceofdifference.Thebestdossagewas100mg/pig,uSittodofieldtriehenThstudiesofere7、sultdemonsdtratethatconimmunostimulcellandhumora1-immunKeyword:comridespoUen—polysacchaimmunosantate引言使用疫苗进行特异性免疫是目前在实际生产中最常用的主要免疫方法,种方法具有针对性,对某些疾病是行之有效的。但也正是这一点,使其应月有〜定的局限性,因为在实际生产中人们不可能对每一种疾病都进行特异f疫,同时由于某些疫苗固有的缺点,其所产生的特异性免疫也不尽理想非特异性免疫的研宄越来越
2、%、2对照组(15.91%),其差异显著(P<0.OS);(3)T淋巴细胞转验组的T淋巴细胞转化率(55.32°%、5639%、54.27°%)均高于对照组及未加PHA组1.34%,其差异显著(p3、haStudiesoeffectnmmuneResponsetoAttenuatedHogcholeraveseCstrainAbstract:Onthisstudyweusedalcoholprecipitationtoextract!ve3509ofposen,andacquired38.89ofcrudeextracts.AfterusedcrudeexffJo(anthraone,mo1ish,se1iwanoff,ioJiJe)reactionacthigpressurliquidchroma1togrtestidentification,canheaphalidati4、onthapolysaccharidesExperimentalgroupswerevacwith①50ragpolySSCChaFidcinatedioOmgpsaccharides+HCLV;③200mgpolysacchm’ides+HCLV;④ph:o1yS01ineS01UtiOn+HCLV;(eVeygrouhavthreenon—immpigs)every'neuneabstraction,respectively;nj^gdetemincell-.immunityedantibodylevelofexperiigherestir5、ive-Erossetcmoralmenta]diffe»fexp)up15.91transfc32%,56.3oughtroupassCD8anta1gria1theistandard.Theresushowed①inRshancontrolgroup.Thougtlymphocyteactive—Erosett:Thehancontrolgrcgroupare:21.84°/25.46%21.92%allhigheest(P<0.#5)significanceofdefference;③Tlymphocytetransformationrateofexperimentalgr6、oupare55.herthancontrolgro47.00%,andnotaddPHAgroup1,34%Thrup口<0.05)signigieanceofdefference;④TiymphocysubgteexperimentalgroupsofavergevalueT1CD3、CIM>hamhatofcontrolgroups,buttheNK1cellofexperime01groupwerenosignificanceofdifference.Thebestdossagewas100mg/pig,uSittodofieldtriehenThstudiesofere7、sultdemonsdtratethatconimmunostimulcellandhumora1-immunKeyword:comridespoUen—polysacchaimmunosantate引言使用疫苗进行特异性免疫是目前在实际生产中最常用的主要免疫方法,种方法具有针对性,对某些疾病是行之有效的。但也正是这一点,使其应月有〜定的局限性,因为在实际生产中人们不可能对每一种疾病都进行特异f疫,同时由于某些疫苗固有的缺点,其所产生的特异性免疫也不尽理想非特异性免疫的研宄越来越
3、haStudiesoeffectnmmuneResponsetoAttenuatedHogcholeraveseCstrainAbstract:Onthisstudyweusedalcoholprecipitationtoextract!ve3509ofposen,andacquired38.89ofcrudeextracts.AfterusedcrudeexffJo(anthraone,mo1ish,se1iwanoff,ioJiJe)reactionacthigpressurliquidchroma1togrtestidentification,canheaphalidati
4、onthapolysaccharidesExperimentalgroupswerevacwith①50ragpolySSCChaFidcinatedioOmgpsaccharides+HCLV;③200mgpolysacchm’ides+HCLV;④ph:o1yS01ineS01UtiOn+HCLV;(eVeygrouhavthreenon—immpigs)every'neuneabstraction,respectively;nj^gdetemincell-.immunityedantibodylevelofexperiigherestir5、ive-Erossetcmoralmenta]diffe»fexp)up15.91transfc32%,56.3oughtroupassCD8anta1gria1theistandard.Theresushowed①inRshancontrolgroup.Thougtlymphocyteactive—Erosett:Thehancontrolgrcgroupare:21.84°/25.46%21.92%allhigheest(P<0.#5)significanceofdefference;③Tlymphocytetransformationrateofexperimentalgr6、oupare55.herthancontrolgro47.00%,andnotaddPHAgroup1,34%Thrup口<0.05)signigieanceofdefference;④TiymphocysubgteexperimentalgroupsofavergevalueT1CD3、CIM>hamhatofcontrolgroups,buttheNK1cellofexperime01groupwerenosignificanceofdifference.Thebestdossagewas100mg/pig,uSittodofieldtriehenThstudiesofere7、sultdemonsdtratethatconimmunostimulcellandhumora1-immunKeyword:comridespoUen—polysacchaimmunosantate引言使用疫苗进行特异性免疫是目前在实际生产中最常用的主要免疫方法,种方法具有针对性,对某些疾病是行之有效的。但也正是这一点,使其应月有〜定的局限性,因为在实际生产中人们不可能对每一种疾病都进行特异f疫,同时由于某些疫苗固有的缺点,其所产生的特异性免疫也不尽理想非特异性免疫的研宄越来越
5、ive-Erossetcmoralmenta]diffe»fexp)up15.91transfc32%,56.3oughtroupassCD8anta1gria1theistandard.Theresushowed①inRshancontrolgroup.Thougtlymphocyteactive—Erosett:Thehancontrolgrcgroupare:21.84°/25.46%21.92%allhigheest(P<0.#5)significanceofdefference;③Tlymphocytetransformationrateofexperimentalgr
6、oupare55.herthancontrolgro47.00%,andnotaddPHAgroup1,34%Thrup口<0.05)signigieanceofdefference;④TiymphocysubgteexperimentalgroupsofavergevalueT1CD3、CIM>hamhatofcontrolgroups,buttheNK1cellofexperime01groupwerenosignificanceofdifference.Thebestdossagewas100mg/pig,uSittodofieldtriehenThstudiesofere
7、sultdemonsdtratethatconimmunostimulcellandhumora1-immunKeyword:comridespoUen—polysacchaimmunosantate引言使用疫苗进行特异性免疫是目前在实际生产中最常用的主要免疫方法,种方法具有针对性,对某些疾病是行之有效的。但也正是这一点,使其应月有〜定的局限性,因为在实际生产中人们不可能对每一种疾病都进行特异f疫,同时由于某些疫苗固有的缺点,其所产生的特异性免疫也不尽理想非特异性免疫的研宄越来越
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