欢迎来到天天文库
浏览记录
ID:13013087
大小:18.85 MB
页数:54页
时间:2018-07-20
《玉米花粉多糖对猪瘟弱毒苗免疫效果的影响研究》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、广西大学硕士学位论文玉米花粉多糖对猪瘟弱毒苗免疫效果的影响研究姓名:温纳相申请学位级别:硕士专业:传染病学与预防医学指导教师:陆芹章2000.6.1摘要本研究采用醇沉水法提取玉米花粉3509,获得粗提复合物38.39。经用蒽酮反应、a萘酚反应、间苯二酚反应、碘化反应及高效液相色谱法鉴定,证实为多糖。/用上述多糖按低剂量(50mg)、中剂量(100mg)、高剂量(200mg)分别与猪瘟弱毒细胞冻干苗共同免疫4组非猪瘟免疫猪,每组3头,每隔7天采血,测定体液免疫和细胞免疫水平。其结果表明:(1)间接血凝试验测得各剂量组的IHA抗体效
2、价均高于对照组,其差异显著(P<O.05);(2)T细胞活性E花环试验,测得各剂量的活性E花环百分率(2184%、25.46%、21.92%)均高于对照组(15.91%),其差异显著(P<0.0S);(3)T淋巴细胞转化试验,测得各试验组的T淋巴细胞转化率(55.32%、5639%、54.27%)均高于对照组(47.00%)及未加PHA组1.34%,其差异显著(p<o.05);(4)T淋巴细胞亚群测定:各试验组的CD3、CD4、CD8T细胞的测定平均值均明显高于对照组,但试验组与.,一对照组的NK细胞测定值差异不显著。厂4根据试
3、验结果选取中剂量在两个猪场进行田间扩大试验。各组测得的IHA抗体效价亦高于对照组,其结果与实验室试验相符合。研究结果表明:玉米花粉多糖既可增强体液免疫,又能增强细胞免疫。关键词:玉米花粉多糖、体液免疫、细胞免疫、免疫增强剂Studiesoneffectofcornpollen-polysaccharidesforimmuneResponsetoAttenuatedHogcholeravirusChineseCstrainAbstract:Onthisstudyweusedalcoholprecipitationtoextra
4、ctive3509ofposen,andacquired38.89ofcrudeextracts.cornAfterusedcrudeexffactdo(anthraone,molish,seliwanoff,iodide)reactionandhighpressureliquidchromatographtestidentification,canvalidationthatit"SpolysaccharidesExperimentalgroupswerevaccinatedwith①50ragpoly
5、saccharides+HCLV;②100mgpolysaccharides+HCLV;③200mgpolysacchm'ides+HCLV;④physiologicalsolinesolution+HCLV;(eveygrouphavethreenon-immunepigs)everyweekabstraction,respectively;thendeteminecell-・immunityandhumoral--immunitystandard.TheresuRsshowed①inktest:an
6、tibodylevelofexperimentalgroupallhigherthancontrolgroup.Thoughttestif'<0.0s)significanceofdifference②Tlymphocyteactive-Erosettetest:Thepersentof们five-Erossettofexperimentalgroupare:21.84%25.46%,21.92%allhigherthancontrolgroup15.91%.Throughttest(P<O.05)signi
7、ficanceofdefference;③Tlymphocytetransformationtest:thetransformationrateofexperimentalgroupare55.32%,56.390,54.27%allhigherthancontrolgroup47.00%,andnotaddPHAgroup1.34%.Throughttest口<0.05)signigieanceofdefference;④Tlymphocytesubgroupassay:theexperimentalgrou
8、psofavergevalueTlympocyteCD3、CIM、CD8allhigherthamthatofcontrolgroups,buttheNKcellofexperimentalgroupsandcontrolgroupwerenotsignifi
此文档下载收益归作者所有