维生素d结合蛋白结构序列预测

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1、维生素D结合蛋白结构预测(—)维生素D结合蛋白(VitaminD-bindingprotein)序歹ij>sp

2、Q3MHN5

3、VTDB_BOVINVitaminD-bindingprotein0S=BostaurusGN=GCPE=2SV=1MKRILVFLLAVAFVHALERGRDYEKDKVCKDLASLGREDFTSLSMVLYSRKFPSGTFEQISHLVNEWSLTVTCCAEGADPDCYDNRTSALSDKSCESNSPFPVHPGTPECCTHEGLEKKLCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKDFADRFMYEYSINYGQA

4、PLTLLVGYTKSYLSMVGSCCTSPNPTVCFLKERLQLKHFSLLTIMTNRICSQYAAYGKEKSRLSHLIKFAQKVPTAHLEDVLPLAEDITTILSKCCDSVSEDCIKELPEYAVKLCDNLSTKNSKFKDCCQEKTPMEIFVCAYFMPASPNPELPDVKLPMNKDVCDEGNTKVLDQYIFELSRKTQTPEVFLTKILESTLKSLDECCHSESSTACLNAKGPQLTRELSSFIQKGQELCADYSENTFTEYKKKLAERLRGKFPDATETDLQELVAKRSDFASKCCSVNSPPLY

5、CNSEIDAEINTLQS(二)理化性质预测1.等电点(pi),相对分子质量(MW)计算Computepl/MwTheoreticalpl/Mw(average)fortheuser-enteredsequence:10MKRILVFLLA20VAFVHALERG30RDYEKDKVCK40DLASLGREDF50TSLSMVLYSR60KFPSGTFEQI70SHLVNEWSL80TVTCCAEGAD90PDCYDNRTSA100LSDKSCESNS110PFPVHPGTPE120CCTHEGLEKK130LCMAALKHQP140QEFPTYVEPT150NDEICE

6、AFRK160DPKDFADRFM170YEYSINYGQA180PLTLLVGYTK190SYLSMVGSCC200TSPNPTVCFL210KERLQLKHFS220LLTIMTNRIC230SQYAAYGKEK240SRLSHLIKFA250QKVPTMLED260VLPLTkEDITT270ILSKCCDSVS280EDCIKELPEY290AVKLCDNLST300KNSKFKDCCQ310EKTPMEIFVC320AYFMPASPNP330ELPDVKLPMN340KDVCDEGNTK350VLDQYIFELS360RKTQIPEVFL370TKILESTLKS

7、380LDECCHSESS390TACLNAKGPQ400LTRELSSFIQ410KGQELCADYS420ENTFTEYKKK430LAERLRGKFP440DATETDLQEL450VAKRSDFASK460CCSVNSPPLY470CNSEIDAEINTLQSTheoreticalpl/Mw:5.36/53342.02维生素D结合蛋白（Q3MHN5）等电点、相对分子质量预测结果图2.蛋白质参数预测Numberofaminoacids:474Molecularweight:53342.0Theoreticalpi:5.36CSVformatAminoacidcompo

8、sition:AlaArgAsnAspCysGinGluGlyHislieLeuLysMetPheProSerThrTrpTyrValPylSec毛毛毛毛毛dP毛毛毛毛毛毛毛dP毛毛毛毛毛毛毛dP9469924096529679506300533553831308145860350010678850497099272107500厶1122141153224312M-777/7M-J-M*77ARNDCQEGHILKMFPSTWYVOU/Vzzvz«/Vzzvz/V/V/V/Vzvzvzvz/Vzv(B)00.0%(Z)00.0%(X)00.0%To

9、talnumberofnegativelychargedresidues(Asp+Glu):68Totalnumberofpositivelychargedresidues(Arg+Lys):552343369961173337Atomiccomposition:CarbonCHydrogenHNitrogenNOxygen0SulfurSFormula:C2343H3€99N€llO733S37Totalnumberofatoms:7423Extinctioncoefficients:Thisproteindoe