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1、ABSTRACT(479-1-1,479-2-1,479-2-2,479-2-3,479-3-1,479-3-2,479-3-3,479-3-4)andX482(482-1-2,482-1-3,482-2-2,482-2-3,482-3-1,482-3-2,482-3-3,482-4-1,482-4-3)wereidentified.PCR-basedmolecularmarkersfromhomologousgroup1and4analysisindicatedthatX479lackofbothchromosomes1Bbandsand4Bspecificbands,but
2、introducedStspecificbandsofhomoeologousgroup1andJsspecificbandsofhomologousgroup4.GliadinandglutelinanalysisshowedthelackofBgenomespecificproteinband.Moreover,FISHbytheBgenomespecificprobesPHVG38andDgenomespecificprobespAs1,alsoshows1Band4BchromosomeswereabsentinX479.PCRamplificationofX482,r
3、evealedthelackofchromosomes4Dand6Dspecificbandsanalyzedbyusingmolecularmarkersfromhomologousgroup4and6.HowevertheJsspecificbandsofhomoeologousgroup4andStspecificbandsofhomologousgroup6werefoundinX482.TheFISHbyDgenomespecificprobespAs1alsodemonstratedX482waslackofchromosomes4Dand6D.Chromosome
4、numbersofX479andX482displayedhighresistanttostriperust.Therefore,X479wasa1St(1B)+4Js(4B)doublesubstitutionlines,whileX482wasa6St(6D)+4Js(4D)doublesubstitutionlines.Insummary,thenewtypeofwheat-Thinopyrumgeneticresourcesidentifiedbymolecularcytogeneticmethods,willbeimportantvaluetowheatgenetic
5、sandbreeding.Keywords:Wheat,Th.intermediumssp.trichophorum,Additionlines,Substitutionlines,StriperustresistanceIV万方数据目录目录第一章前言..........................................................11.1偃麦草遗传资源的研究...........................................11.2偃麦草的分类分布及植物生物学、生态学特性.......................11.2
6、.1偃麦草的分类分布...........................................11.2.2偃麦草的植物学特性.........................................11.2.3偃麦草的生态学特性.........................................21.3偃麦草染色质向小麦遗传背景的导入...............................21.3.1外源染色质导入小麦的方法...................................21.3.2小麦背景中外源染色质的检测....
7、.............................31.3.3小麦异染色体系的选育.......................................51.4偃麦草的抗病性.................................................7第二章材料与方法....................................................82.1实验材料...................................
