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ID:22960728
大小:615.31 KB
页数:22页
时间:2018-11-02
《海南大学植物保护专业毕业论文(设计)》由会员上传分享,免费在线阅读,更多相关内容在工程资料-天天文库。
1、东寨港红树林吸血蠓的物种多样性摘要红树林是生长在热带、亚热带海岸潮间带或河流入海口的木木植物群落,是适应陆地与海洋过渡带的一种特殊森林类型。由于其独特的海陆过渡特性,红树林具有极大的社会、经济、生态价值。红树林作为重要的海岸湿地类型是国际上湿地生态保护和生物多样性保护的重要对象。吸血蠓是一类微小型昆虫,隶属双翅0,蠓科。奋吸血性的蠓侵袭人畜,传播疾病,作为病原体的宿主,是一类重要的医学昆虫。采用传统的形态学分类特征对吸血蠓进行鉴定极易混淆,更难进行地理分布与遗传多样性分析,近年来迅速发展的分子鉴技术为解决吸血蠓遗传及鉴定等问题提供了有效的手段。本论文综合利用网捕
2、、人诱和灯诱的方法对海南东寨港红树林的吸血蠓类进行系统采集,基于形态共成功鉴定出6种库蠓,包括棒须库蠓Culicoidesclavipalpis、环基库蠓Culicoidescircumbasalis>霍飞库蠓Culicoideshuffi、尖嗡库蠓Culicoidesoxystoma>帛琉库蠓Culicoidespeliliouensis和Culicoidesornatus种团;系统研究了6种库蠓翅的色斑类型、翅脉和翅室的异同,并根据翅的特征编写了检索表;此外,成功测定了6种库蠓的DNA条形码基因COI的基因序列。该研宄为吸血蠓类昆虫的分子鉴定奠定丫基础,科为
3、蠓类昆虫的防治提供理论依据。关键词:吸血蠓;形态鉴定;分子鉴定;DNA条形码;红树林AbstractMangroveisgrowthintropicalandsubtropicalcoastintertidalzoneorrivermouthofwoodyplantcommunity,istoadapttothelandandoceantransitionzoneisakindofspecialforesttypes.Becauseofitsuniquesea-landtransitioncharacteristicsofmangroveisofgreatsoc
4、ial,economicandecologicalvalue.Mangroveasanimportantcoastalwetlandtypesareinternationalwetlandconservationandbiodiversityprotectionofimportantobjects.Vampiremidgeisakindofsmallinsects,diptera,midge.Midgeaffecthumanandanimalbloodsuckinghabits,diseasetransmission,asthehostofpathogens,i
5、sakindofimportantmedicalinsects.Usingtraditionalmorphologicalclassificationfeatureofvampiremidgeconfusingverification,hardertogeographicaldistributionandgeneticdiversityanalysis,inrecentyearstherapiddevelopmentofmolecularlearningtechniquestosolvethevampiremidgegeneticprovidesaneffect
6、ivewayandtheproblemofidentification.Comprehensiveutilizationofthetrap,thispaperinducedandtrapmethodtotheportofHainanDongzhaigangmangrovevampiremidgesystematicallycollected,basedonmorphologyweresuccessfullyidentifiedsixmidge,includingCulicoidesclavipalpis,Culicoidescircumbasalis,Culic
7、oideshuffi,Culicoidesoxystoma,CulicoidespeliliouensisandCulicoidesomatusgrr?n/?;Systemstudiedthe6typesoflibrarymidgewingspots,veinandwingrooms,aswellasthesimilaritiesanddifferencesofandaccordingtothecharacteristicsofthewingstowritetheretrievaltable;Inaddition,thesuccessfuldeterminati
8、onof6kindsof
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