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1、连接互相匹配的粘性末端，产生新的酶切位点通常连接互相匹配的粘性末端可以产生新的酶切位点。以下组合是通过计算机程序计算出来的，尽管我们希望能尽量保证其准确性，但有些未经实验证实，因此不能保证100%的可行性。如果有同裂酶存在，则只列出其中的一个酶。本表中列出的内切酶均为已商品化的内切酶。识别序列不够严谨的内切酶（例如，识别多个序列的酶）只在括号内标出相关的序列，但注意这些酶不只切割该序列。"-"表示连接产物不能被再切割。酶连接到可进行切割的酶Acc65I(G/GTACC)BanI(G/GTACC)Acc65I,BanI,K
2、pnI,NlaIV,RsaI BsiWI,BsrGIRsaIAccI(GT/CGAC)AciI,AclI,BsaHI(GR/CGYC),HinP1I,HpaII,NarI--(GT/CGAC)ClaI,BstBI,TaqITaqIAciI(C/CGC)AccI(GT/CGAC),AclI,ClaI,BstBI,TaqI--BsaHI(GR/CGCC),HinP1I,NarIAciIHpaIIHpaIIAclI(AA/CGTT)AccI(GT/CGAC),AciI,ClaI,BstBI,HinP1I,HpaII,NarI,
3、TaqI--AflIII(A/CGCGT)AscI,BssHIIBstUI,HhaI(A/CATGT)BspHI,NcoI,PciINlaIII(A/CGCGT)MluIAflIII,BstUI,MluIAgeI(A/CCGGT)AvaI(C/CCGGG),XmaIHpaII,NciI,ScrFIBsaWI,BspEIBsaWI,HpaIIBsrFI(A/CCGGT),SgrAI(CA/CCGGTG)AgeI,BsaWI,BsrFI,HpaIINgoMIVBsrFI,HpaIIApaI(GGGCC/C)BanII(GGG
4、CC/C),Bsp1286I(GGGCC/C)ApaI,BanII,Bsp120I,Bsp1286I,HaeIII,NlaIV,Sau96IApaLI(G/TGCAC)SfcI(C/TGCAG)BsgIApoI(A/AATTY)EcoRIApoI,Tsp509I(G/AATTY)EcoRIApoI,EcoRI,Tsp509I(R/AATTY)MfeI,Tsp509ITsp509IAscI(GG/CGCGCC)AflIII(A/CGCGT),MluIBstUI,HhaIBssHIIBssHII,BstUI,Cac8I,Hh
5、aIAseI(AT/TAAT)BfaI,Csp6I,NdeI--MseIMseIAsiSI(GCGAT/CGC)BsiEI(CGAT/CG)DpnII,PvuIPacIMseIPvuIDpnII,PvuIAvaI(C/CCGGG)AgeI,BsaWI,BspEI,BsrFI(R/CCGGY),NgoMIV,SgrAI(CR/CCGGYG)HpaII,NciI,ScrFI(C/TCGAG)XhoIAvaI,TaqI,XhoI(C/TCGAG)SalITaqI(C/CCGGG)XmaIAvaI,BsaJI,HpaII,Nci
6、I,ScrFI,SmaIAvaII(G/GWCC)PpuMI(RG/GACCY)AvaII,NlaIV,Sau96IRsrIIAvaII,Sau96IPpuMI(RG/GTCCY)AvaII,BsmFI,NlaIV,Sau96IAvrII(C/CTAGG)NheI,SpeI,XbaIBfaIStyI(C/CTAGG)AvrII,BfaI,BsaJI,StyIBamHI(G/GATCC)BclI,DpnIIAlwI,DpnIIBglII,BstYI(R/GATCY)AlwI,BstYI,DpnIIBstYI(G/GATCC
7、)AlwI,BamHI,BstYI,DpnII,NlaIVBanI(G/GTACC)Acc65IAcc65I,BanI,KpnI,NlaIV,RsaI(G/GCGCC)KasIBanI,BsaHI,HaeII,HhaI,KasI,NarI,NlaIV(G/GTACC)BsiWI,BsrGIRsaIBanII(GGGCC/C)ApaI,Bsp1286I(GGGCC/C)ApaI,BanII,Bsp1286I,HaeIII,NlaIV,Sau96I(GAGCT/C)Bsp1286I(GAGCT/C),SacIAluI,Ban
8、II,BsiHKAI,Bsp1286I,SacIBclI(T/GATCA)BamHI,BstYI(R/GATCY)AlwI,DpnIIBglII,MboIIDpnIIBfaI(C/TAG)AseI,Csp6I,MseI,NdeI--BglII(A/GATCT)BamHI,BstYI(R/GATCY)AlwI,BstY
