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1、土壤中脱氢酶活性的测定殷晓晨,武亨杰,朱承彬(中国石油大学化学化工学院,山东青岛266555)摘要:为了研究土壤中微生物降解有机物的能力,设计实验,采用TTC分光光度法测定受石油污染并接受生物修复的各土壤样品中脱氢酶的活性。研究表明,所采集的土壤样品中脱氢酶的含量很少,基本无法用本方法检测出,说明土壤在过去的一年里发生了某些变化使脱氢酶含量降低。关键词:土壤;脱氢酶;TTC;紫外分光光度法中图分类号:X705文献标志码:AMeasurementofCatalaseActivityinSoilYinXiao-chen,WuHeng-jie,ZhuCheng-bin(CollegeofCh
2、emistryandChemicalEngineering,ChinaUniversityofPetroleum,Qingdao266555,China)Abstract:Thisexperimentisdesignedtoinvestigatetheabilityofmicroorganismsinsoiltodegradeorganicmatters.Thedehydrogenaseactivityinsoilwhichwascontaminatedbypetroleumandthenbiologicallyrepairedwasmeasuredbythemethodofultra
3、violetspectrometry.Theresearchshowsthatthecontentofdehydrogenaseinsoilssamplesaresominutethatcannotbedetectedbythismethod.Keywords:soil;dehydrogenase;TTC;ultravioletspectrometry.school)],44housebuildingaswellasmorethan1000copiesofbooks,morethan10kindsofcollectionofnewspapersandmagazines,firesbur
4、ntdown.In1938,theJapaneselearnedthatthenationalGovernmentestablishedinXITangTombrotten"clip"taxes,taxofficerslivein8ofYanDongvillagefarmershome,troopsatnight,Yaowasburnedseveralhouses.1938newmorningofSeptember18,theJapaneseteamwenttothecountryside"sweep"fromthekaiyangvillagelinetoTungYangJiaQiao
5、yanTemplewallandmetYangJia溇ZhouDana(male)weeksbecauseoflong-termillnessandcan'tworkinthefields,theJapaneseseehisfacefromscratchwithoutcalluses,regardhimas"Shina",thrustaknifeatthesceneofhisdeath.Thebodywasstabbed7times,wound13.Thesameday,TrangbangvillageheardtheJapanesecometo"mop-up"arehiding,mo
6、therof9ZhouGuanbaoZhangAyingtodrilla"drymound",wasdiscoveredbytheJapanese,ashotintheend.Thesameday,is7zhouhaijiangzhigrandfatherZhouYingbaotoescapetheJapanesearmy,wasfoundontheroad,ashotintheend.InMarch1939,theJapanesearmyinYanTombraiding,hasarrested16people,infengqiaocigarettesthismorningbang,k
7、illing11peopleontheRiver,Eastmeetingpointinwoodqiaotuintheafternoonkilling4peopleontheRiverside.GuTongisaJapanesegospeaktheShanghaidialect,latertobecalledGuTongas"killleft."OnJanuary18,1943,theelves,fivethousandorsixthousand