不同剂量胰岛素对严重烫伤大鼠早期血清il

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1、不同剂量胰岛素对严重烫伤大鼠早期血清IL王耘川，高峰，贾赤宇，张万福，汤朝武，胡大海【关键词】烧伤；胰岛素；炎症；细胞因子类　　RegulatingeffectsofdifferentdosesofinsulinonserumIL1βandIL10inearlystageinseverelyscaldedrats　　【Abstract】AIM:TodeterminethepossibleeffectsofdifferentdosesofinsulinontheproinflammatorycytokineIL1βandtheantiinflammatorycytokineIL1

2、0inearlystageofsevereburninrats.METHODS:MaleSpragueDalydividedinto2groupstoreceiveeithernormalsalineonly(n=14,L/kgnormalsaline,groupA)ornormalsalineplusdifferentdosesofinsulin(n=42,L/kgnormalsalineplussubcutaneousinjectionof1,3or5IU/kginsulinrespectively,groupB1,B2,B3).Inaddition,7ratsalinju

3、ryalcontrolgroup(groupN).Theoutemeasuresenpassedtheserumglucose,bloodpressure,serumIL1βandIL10inratsat6and24hafterscaldanddifferenttreatments.RESULTS:Theserumglucoseincreasedquicklyinratsafterscald,onstratedingroupAtoarangeof(10.7±1.8)mmol/Lby6hpostscald.Insulindecreasedtheserumglucoseinados

4、edependentmannerinratsofgroupB1,B2andB3.Andinsulinatadoseof3IU/kgbodyglucosetoarangeof(6.0±0.8)mmol/Linratsat6hafterthermalinjury,osteqealtothenormalrangeof(5.1±0.6)mmol/L(P>0.05).Meanprovedthebloodpressureofscaldedratsbykeepingthebloodpressureatastableconditionsimilartothenormal.Insulins

5、ignificantlydecreasedtheserumIL1βinadosedependentmannerinscaldedrats.Itinistrationofinsulinof3IU/kgserumIL1βuchloinistrationinratsat6and24hafterthermalinjury(P0.01).InsulincouldincreasetheserumIL10inscaldedrats.InparisonostsignificantincreaseofserumIL10ightattenuatetheinflammatorycascaderesp

6、onsebydecreasingtheproinflammatorycytokineIL1βandincreasingtheantiinflammatorycytokineIL10,therebykeepingorrestoringthesystemichomeostasisaftersevereburn.Thebestadministrationdoseofinsulininearlyburnstageshouldbedeterminedbynormalizingserumglucose.　　【Keymation;cytokines　　【摘要】目的：研究不同剂量胰岛素对严重烫

7、伤早期血清IL1β及IL10的影响.方法：将雄性SD大鼠63只随机分为烫伤后盐水治疗（A）组、盐水+不同剂量胰岛素治疗（B1~B3）组，每组14只，另取7只正常大鼠作为对照（N）组.烫伤各组在背部造成约30%体表总面积（TBSA）全层皮肤烫伤.伤后6，24h后检测血糖、血压及血清IL1β及IL10含量.结果：伤后大鼠血糖明显升高，6h达到（10.7±1.8）mmol/L，并持续高血糖状态，胰岛素可以降低伤后血糖，并随剂量的增大而作用增强，3IU/kg胰岛素的6h血糖为(6.0±0.8）mmo