αb―crystallin在喉癌组织中的表达及临床意义

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1、aB—crystallin在喉癌组织中的表达及临床意义［摘要］目的探讨c(B-crystallin基因在喉癌组织中的表达及其与喉癌的关系。方法收集2008年3月〜2014年10月南京医科大学第二附属医院及皖南医学院弋矶山医院手术切除的喉癌标本54例及13例癌旁正常黏膜标本，采用实时荧光定量PCR(Real-timePCR)检测8例喉癌组织及其癌旁正常组织中aB-crystallin基因的表达，免疫组化法检测54例癌组织及13例癌旁正常组织中ctB-crystallin蛋白的表达，比较不同组织中ctB-crystallin阳性表达率。结果喉癌组织

2、中的aB-crystallinmRNA表达量及aB-crystallin蛋白阳性表达率均明显高于癌旁正常组织，差异均有高度统计学意义(P0.05)。结论aB-crystallin基因的高表达与喉癌发生发展及转移有关，可能为喉癌患者的预后提供一个潜在的风险评估指标。［关键词］aB-crystallin；实时荧光定量PCR;喉癌；免疫组化［中图分类号］R739.63［文献标识码］A［文章编号］1673-7210(2016)10(a)-0009-04ExpressionandsignificanceofaB-crystallininlaryngeal

3、squamouscellcarcinomaXIONGLinlMAOYuan2MAJun3ZHANGDawei4AZHUJin5CHENRenjie41.DepartmentofPathology，theSecondAffiliatedHospitalofNanjingMedicalUniversity，JiangsuProvince，Nanjing210029，China；2.DepartmentofOtolaryngology-HeadandNeckSurgery，JiangsuProvincialOfficialHospital，Jiang

4、suProvince，Nanjing210024，China；3.DepartmentofOtolaryngology-HeadandNeckSurgery，YijiShanHospitalofWannanMedicalCollege，AnhuiProvince，Wuhu241001，China；4.DepartmentofOtolaryngology-HeadandNeckSurgery,theSecondAffiliatedHospitalofNanjingMedicalUniversity,JiangsuProvince，Nanjing2

5、10029，China；5.MilitaryMedicalResearchInstituteofNanjingMilitaryRegion，JiangsuProvince，Nanjing210002，China[Abstract]ObjectiveToexaminetheexpressionofaB-crystallininhumanlaryngealsquamouscellcarcinoma(LSCC)andinvestigatetherelationshipbetweenitsexpressionandLSCC.MethodsAtotalo

6、f54casesofLSCCand13casesofadjacentnormaltissueswerecollectedfromtheSecondAffiliatedHospitalofNanjingMedicalUniversityandYijiShanHospitalofWannanMedicalCollegefromMarch2008toOctober2014.Real-timequantitativepolymerasechainreaction(8LSCCtissues，8adjacentnormaltissues)andimmuno

7、histochemistry(54LSCCtissuesand13adjacentnormaltissues)wereperformedtodetectedtheexpressionofaB-crystallingeneandproteininLSCC.ThepositiveexpressionrateofaB-crystallinindifferenttissueswascompared.ResultsThepositiveexpressionrateofaB-crystallinmRNAandproteininLSCCweresignifi

8、cantlyhigherthanthoseintumor-adjacentnormaltissues,thedifferenceswerestatis