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1、MPP合剂急性毒性试验及其促睡眠作用王乐1正阳2孙辉2杭景2曾建3狄荣科3通讯作者1.江苏大学医学院临床医学专业2010级；2.江苏大学医学院临床医学专业2011级；3.江苏大学医学院江苏镇江212013【摘要】目的：观察MPP合剂促进睡眠的作用及其安全性。材料与方法：釆用最大耐受剂量法行急性毒性试验。一个月龄纯种ICR小鼠60只，分为实验组(MPP组)和对照组(NS组)，每组30只，雌雄各半；2组分别灌胃MPP合剂或生理盐水lmg,连续30天；观察一般状况，测量试验期初、期末体重，观察并记录小鼠入睡率，取材作组织病理学观察。结果：2组小鼠在相同的环境和喂饲条件下，一般状况
2、无明显差异；比较MPP组和NS组试验期初和期末体重没有显著差异(p=0.552)；组织病理学观察，与NS组相比MPP组小鼠的肝脏、肾脏、脑和睾丸未见发生病理变化。观察小鼠入睡率，MPP组为76%,NS组为20%,MPP合剂明显提高小鼠入睡率(p=0.009)。结论：MPP合剂能有效促进睡眠和提高睡眠质量，未见对肝、肾、脑和睾丸毒性，属安全无毒级保健药品。【关键词】褪黑激素；珍珠粉；急性毒性试验；促睡眠作用【中图分类号】R282.71【文献标识码】B【文章编号】1674-8999(2015)8-0197-02Atestabouttheacutetoxicityandsleep
3、promotingeffectofMPPmixtureWangLeiBaoZhengyang2SunHui2HangJing2ZengJian3DiRongke3*(1.ClinicClass2010ofMedicalSchoolinjiangsuUniversity,2.ClinicClass2011ofMedicalSchoolinjiangsuUniversity,3.MedicalSchoolofJiangsuUniversity;JiangsuZhenjiang,212013)【Abstract】Objective：Toobservetheeffectandsaf
4、etyofMPPmixtureonpromotingsleep.Materialandmethods：Themaximumtolerateddosemethodofacutetoxicitytestwasusedinthisstudy.60onemontholdpurebredICRmiceweredividedintoexperimentalgroup(groupMPP)andcontrolgroup(NSgroup),30miceineachgroupwithhalfmaleandhalffemale.Twogroupswereorallyfedforonemonth,
5、thegeneralconditionandbodyweightwereobservedandmeasured,thetissuesofliver,kidney,brain,andtestisweretakenforhistopathologicalobservationundermicroscope.Results：Underthesameenvironmentandfeedingconditions,nosignificantdifferenceingeneralconditionwereobservedbetweenthetwogroupmice;therewasno
6、significantdifferenceofbodyweightbetweenMPPgroupandNSgroupattheearlyandendoftheexperimentalpiled(p=0.552);byhistopathologicalobservation,comparedwithNSgroup,theliver,kidney,brainandtestisofMPPgroupmicedidnotfindpathologicalchanges.But,thesleepingratewas76%inMPPgroupand20%inNSgroup,therewas
7、significantlyimprovethesleeprateofMPPmixture(p=0.009).Conclusion：MPPmixturecaneffectivelypromotesleepandimprovesleepquality,notontheliver,kidney,brainandtestistoxicity,issafenon-toxicgradehealthcaremedicine.【keywords】melatonin;pearlpowder;acutetoxicitytest;sle
