高密度脂蛋白抗动脉粥样硬化机制—SR-BI, CD36, PPARgamma的作用PPT课件.ppt

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1、高密度脂蛋白抗动脉粥样硬化机制—SR-BI,CD36,PPARgamma的作用南华大学心血管疾病研究所易光辉1泡沫细胞形成是动脉粥样硬化发病的核心2SR-BI3动脉粥样硬化小型猪SR-BI、CD36和PPARγ表达的变化第一部分4Figure1:Themeanbodyweightsofthetwogroupsthroughoutthestudy动脉粥样硬化小型猪体重的变化5Figure2:Plasmaleveloftotalcholesterol(a),HDL-cholesterol(b)andtriglyceride(c)inminiswine动脉

2、粥样硬化小型猪血脂水平的改变6ABFigure3:Highfat/highcholesteroldiet-inducedatherosclerosisinminiswine.A:photographofpinned-outcoronaryarteryincontrolfedpigs;B:photographofpinned-outcoronaryarteryinHFHC-fedpigs.动脉粥样硬化小型猪冠状动脉苏丹Ⅳ染色7As小型猪主动脉HE染色(×20)对照组实验组8ABFigureHighfat/highcholesteroldiet-indu

3、cedatheroscleroticplaquewasstainedwithhematoxylineosininminiswine.Magnificationis×40A:sections(5µmthick)weretakenattheabdominalaortaincontrolfedpigs;B:sections(5µmthick)weretakenattheabdominalaortainHFHC-fedpigs.9ABFigure5:Highfat/highcholesteroldiet-inducedfattyliverwasstained

4、withhematoxylineosininminiswine.Magnificationis×40A:sections(5µmthick)weretakenattheliverincontrolfedpigs;B:sections(5µmthick)weretakenattheliveinHFHC-fedpigs.动脉粥样硬化小型猪动脉及肝脏HE染色10*GAPDHSR-BIM1234Figure6:ExpressionofSR-BImRNAinminiswinefedwithHFHC1:Liverincontrolgroupminiswine;2

5、:LiverinminiswinefedwithHFHC;3:Aortaincontrolgroupminiswine;4:AortainminiswinefedwithHFHC;M：Markers；*:P<0.05,comparedwithcontrolgroup.（n=5)SR-BImRNA在动脉粥样硬化小型猪组织表达的变化111234Figure7:ExpressionofSR-BIproteininminiswinefedwithHFHC.(n=5)1:Liverincontrolgroupminiswine;2:Liverinminiswi

6、nefedwithHFHC;3:Aortaincontrolgroupminiswine;4:AortainminiswinefedwithHFHC.*:P<0.05,comparedwithcontrolgroup.动脉粥样硬化小型猪SR-BI蛋白表达的变化12ABFigure8:ExpressionofSR-BIproteininminiswine(×40)(n=5)A:Liverincontrolgroupminiswine;B:LiverinminiswinefedwithHFHC;13M123456GAPDH596bpCD36246bp1:

7、对照组肝脏;3:对照组肾脏;5:对照组主动脉；2:实验组肝脏;4:实验组肾脏;6:实验组主动脉.*P<0.05,与对照组比较500bpAs小型猪组织CD36mRNA的表达变化141234561:对照组肝脏;3:对照组肾脏;5:对照组主动脉；2:实验组肝脏;4:实验组肾脏;6:实验组主动脉.*P<0.05,与对照组比较As小型猪组织CD36蛋白质的表达变化15免疫组织化学方法检测小型猪组织CD36蛋白质的表达A:对照组肝脏;C:对照组肾脏;E:对照组主动脉;B:实验组肝脏;D:实验组肾脏；F:实验组主动脉.CDBAEF16GAPDH697bpPPARγ

8、308bp500bp*P<0.05,与对照组比较MarkerControlHFHCAs小型猪主动脉组织PPA