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ID:20546160
大小:4.21 MB
页数:4页
时间:2018-10-10
《左向右分流型肺动脉高压大鼠肺组织中microrna的》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、左向右分流型肺动脉高压大鼠肺组织中microRNA的表达谱及初步分析卢荣鑫,钟前进(400042重庆,第三军医大学大坪医院野战外科研究所心血管外科)[摘要]目的检测晚期肺动脉高压(pulmonaryhypertension,PH)大鼠肺组织中microRNA(miRNA)的表达,初步预测差异表达的microRNA调控的靶基因。方法4~5周龄健康雄性SPF级SD大鼠20只,体质量90~110g,完全随机分组法将全部大鼠分成分流组(n=10)和对照组(n=10)。分流组采用套管法行右侧颈总动脉-颈外静脉分流术以建立左向右分流型肺动脉高压模型,对照组行假手术,建立左向右分流
2、型肺动脉高压大鼠模型12周后,取大鼠外周肺组织,运用microRNA表达谱芯片检测分流组和对照组大鼠肺组织microRNA的差异性表达。运用Miranda、TargetScan、PicTar软件预测可能调控的靶基因,实时定量PCR验证miR-98、miR-130b和miR-127等的表达。结果平均肺动脉压(mPAP)、肺动脉中膜厚度百分比(MT%)及右心室肥厚指数(RVHI)均明显高于对照组(P<0.01),microRNA芯片结果提示:与对照组相比,在分流组大鼠肺组织中表达明显上调的miRNA有30个(miR-122、miR-130b、miR-146b等),明显下的
3、miRNA有7个(miR-382、miR-192、miR-29c等),RT-PCR结果与芯片结果一致。结论microRNA在左向右分流型肺动脉高压大鼠中表达存在差异,microRNA可能参与肺动脉高压大鼠肺血管的重构。[关键词]肺动脉高压;microRNA;肺血管重构[中图法分类号][文献标志码]ADetectionandanalysisonmicroRNAExpressionProfilesinperipheralpulmonarytissuesofratswithpulmonaryhypertensioninducedbylefttorightshunt[Abst
4、ract]ObjectiveToinvestigatethedifferentialexpressionsofmicrRNAsinperipheralpulmonarytissuesofratswithpulmonaryhypertension(PH)inducedbylefttorightshunt.MethodsTwentymaleSDrats(aged4-5weeks,weighing95-110g)wererandomlydividedintoshuntgroupandcontrolgroup(10each).Ratsinshuntgroupunderwent
5、rightcommoncarotidartery-externaljugularveinconnectiontoreproducePHmodeloflefttorightshunt,whileanimalsincontrolgrouponlyreceivedshamoperation.Theperipheralpulmonarytissueswerecollected12weeksaftertheoperation.Quantitativereal-timePCRwereperformedtoconfirmtheresultsobtainedbymicroarraya
6、nalysis.Meanwhile,theirtargetgeneswerepredictedbymiRNAtargetgenepredictionsoftwaressuchasMIRanda,TargetScanandPicTar.ResultsMeanpulmonaryarterialpressure(mPAP),rightventricularhypertrophyindex(RVHI),percentageofsmallpulmonaryarteriesmediathickness(MT%)weresignificantlyincreasedinshuntgr
7、oupcomparedwithcontrolgroupafter12weeksshunt(P<0.01).MicroRNAmicroassayshowedthat37microRNAsweredifferentiallyexpressedinshuntgroupandcontrolgroupincludingasetof30overexpressedmicroRNAsand7downexpressedmiRNAs.RT-PCRverifiedthatmiR-130b,miR-98wereupregulated,whilemiR-127wasdownr
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