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1、SupplementarvSection2SupplementaryTable:DetailsofMarkersusedintheStudytoCharacterizePluripotentStemCellsandDifferentiatedGermCellsAntibody&DilutionSource&CatalogNumberFunctionalSignificanceStainingPatternAntibodyusedinotherstudiesforsimilarcharacteriz•ationReferenceOCT-4(1/100)Abeam,UKab198
2、57AnoctamerbindingnucleartranscriptionfactorofthePOUfamily,with360aminoacidsusedtodefinepluripotentstateofastemcell,wellstudiedinembryonicstemcell(ES)andcarcinomastemcells.Itisagerm-linespecificmaternallyexpressedfactorexpressedbyPGCsandgermcellsduringembryonicdevelopment.Itiscriticalforear
3、lyembryogenesisandforEScellpluripotency.AlsoknowntobeexpressedinVSELsandtissue-specificprogenitors.Oct-4AisoformisnuclearinpluripotentstemcellsOct-4Bisoformislocalizedincytoplasmofprogenitorsviz.OGSCsandGCNParteetal2011[12][12,13,55-57]SSEA-4(1/50)Millipore,USAMAB4304SSEA-4,aglycolipidcarbo
4、hydrateepitopethatisexpresseduponthesurfaceofhumanembryonic-carcinoma,germandEScells.Identifiesadultmesenchymalstemcellpopulation.AlsoknowntobeexpressedinVSELsandtissue-specificprogenitors.Cellsurfaceinpluripotentstemcellsandminimallycytoplasmicinprogenitorsviz.OGSCsandGCNKumaretal2009,[58]
5、Parteetal2011[12][5,14,59-61]CD133(1/50)Millipore,USAMAB4399A115-120kDamemberofInterferon-induciblepentaspantrans-membranedomainglycoprotein,markstheonsetofgermcellcompetence,alsoexpressedinbraintumours,ahematopoieticandneuronalstemcellmarkerCellsurfaceinpluripotentstemcellsandcytoplasmicin
6、progenitorsviz.OGSCsandGCNpersonalobservation[49-5262-65]FRAGILIS(1/50)Abeam,UKab74699Aninterferoninduciblegenecodingfortrans membraneproteinisthefirstgeneimplicatedinacquisitionofgermcellcompetence,expressedbycellsintheprimitivestreakduringgermlinedevelopmentCellsurfaceinpluripotentstemcel
7、lsandcytoplasmicinprogenitorsviz.OGSCsandGCNBhartiyaetal2011[66][67-6953-55]STELLAMillipore,NovelgenespecificallyexpressedinPGCs,oocytes,Nuclearinpluripotentstempersonal[70-72](1/50)USAMAB4388pre-implantationembryosandpluripotentcells,amaternalfactoresse
