复制（第五讲）ppt课件

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1、免疫学信息网http://www.immuneweb.comFormationofpre-Replicativecomplex免疫学信息网http://www.immuneweb.comReplicationcomplexesduringthecellcycle免疫学信息网http://www.immuneweb.comG2MORCMCMMCMCDC6+Cdt1ReplicationMachinery“Pre-ReplicativeComplex”ORCCDC6+Cdt1SlateG1earlyG1免疫学信

2、息网http://www.immuneweb.comEukaryoticinitiationcomplexORC:AsixsubunitproteincomplexwhichhasbeenimplicatedasbeingtheeukaryoticDNAreplicationinitiatorprotein.Subunitsarenamedaccordingtotheirsize,withORC1beingthelargestandORC6beingthesmallestsubunit.YeastORCsp

3、ecificallybindstoreplicationoriginsinanATPdependentmannerandhasbeenshowntopossessATPaseactivity.CDC6/Cdc18:Anessentialfactorfortheassemblyofthepre-replicativecomplexesthatco-operateswithCdt1toloadMCM2-7Proteolyzedinyeastsorexportedoutofthenucleusinmammalia

4、ncellsattheG1-Stransition.OverexpressionofCdc6inyeastcausesmultipleroundsofDNAreplicationwithoutinterveningmitosis,makingitacriticalregulatorofDNAreplication.MCM2-7:Afamilyofsixrelatedproteins(MCM2-MCM7)whichseemtofunctiontogetherinalargemulti-subunitprote

5、incomplex.TheroleofMCMísinDNAreplicationisstillspeculative,rangingfrommediationofprotein-DNAinteractionstoDNAhelicaseactivity.免疫学信息网http://www.immuneweb.comA/TDUEOREAuxiliaryelementsAuxiliaryelementsOriginrecognitionproteinsTranscriptionFactorsTranscriptio

6、nFactorsLeadingstrLeadingstrLaggingstrLaggingstrCoreoriginOriginsaremulti-partiteTHESTRUCTUREOFARS1(S.CEREVISIAE)免疫学信息网http://www.immuneweb.comB1ACSB2B3ABF1ORC“Post-RC”protection“Pre-RC”protection134564122免疫学信息网http://www.immuneweb.com免疫学信息网http://www.imm

7、uneweb.comORCusesdifferentstrategiesforbindingDNAindifferentspecies免疫学信息网http://www.immuneweb.comPre-RCformationORC:AsixsubunitproteincomplexwhichhasbeenimplicatedasbeingtheeukaryoticDNAreplicationinitiatorprotein.Subunitsarenamedaccordingtotheirsize,withO

8、RC1beingthelargestandORC6beingthesmallestsubunit.YeastORCspecificallybindstoreplicationoriginsinanATPdependentmannerandhasbeenshowntopossessATPaseactivity.CDC6/Cdc18:Anessentialfactorfortheassemblyofthepre-re