构建不同质粒评估抗质粒接合作用(英文版)

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1、ZHENG–CONSTRUCTIONOFREPORTERPLASMIDSFORTHEEVALUATIONOFNATURALPRODUCTSASANTI-PLASMIDAGENTSCONSTRUCTIONOFREPORTERPLASMIDSFORTHEEVALUATIONOFNATURALPRODUCTSASANTI-PLASMIDAGENTSThisdissertationissubmittedinpartfulfilmentoftherequirementsfortheMasterinPharmaco

2、gnosy,DepartmentofPharmaceuticalandBiologicalChemistry,SchoolofPharmacy,UniversityCollegeLondon.CandidateName:ZhengZhejiaoCandidateNumber:14089435Supervisor:Dr.PaulStapletonDate:28/8/2015WordCount:9430IssueDate:24/08/2015Page55of55Revision:BZHENG–CONSTRU

3、CTIONOFREPORTERPLASMIDSFORTHEEVALUATIONOFNATURALPRODUCTSASANTI-PLASMIDAGENTSPLAGIARISMSTATEMENTThisthesisdescribesresearchconductedintheSchoolofPharmacy,UniversityCollegeLondonbetweenMay2015andAugust2015underthesupervisionofDr.PaulStapleton.Icertifythatt

4、heresearchdescribedinthisworkisoriginalandthatanypartsoftheworkthatIhavebeenconductedincollaborationareclearlyindicated.IalsocertifythatIhavewrittenallthetexthereinandhaveclearlyindicatedbysuitablecitationanypartofthisdissertationthathasalreadyappearedin

5、publication.SignedinLondonFriday,August28,2015:(Signature&Candidate’sNameinPrint)ZhengZhejiaoIssueDate:24/08/2015Page55of55Revision:BZHENG–CONSTRUCTIONOFREPORTERPLASMIDSFORTHEEVALUATIONOFNATURALPRODUCTSASANTI-PLASMIDAGENTSACKNOWLEDGMENTSThankstoDr.PaulSt

6、apleton,whoprovidedmethetopicoftheproject.Healsogavemesomeinstructionsduringtheexperimentsanddissertationwriting.ThankstoMr.PedroErnestodeResendeandMissSarahSoares,whotaughtmetheskilloftheexperimentsandhelpedmetofinishthem.Also,thankstoallotherstaffinthe

7、laboratory.IssueDate:24/08/2015Page55of55Revision:BZHENG–CONSTRUCTIONOFREPORTERPLASMIDSFORTHEEVALUATIONOFNATURALPRODUCTSASANTI-PLASMIDAGENTSTABLEOFCONTENTSCONTENTS1.Introduction111.1TherelationshipbetweenPlasmidconjugationandantibioticsresistence111.2Bio

8、luminescenceandLuxoperon141.3Reporterstrainstostudybioactivitiesincludinganti-conjugationassay162.AimsofProject183.MaterialandMethods193.1TheStrains193.2Reagents203.3Equipment213.4Method213.4.1Assay1:Settinguptheanti-conju