玄参和附子抗鼠心肌肥厚作用的对比实验研究.doc

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1、玄参和附子抗鼠心肌肥厚作用的对比实验研究【摘要】目的：探讨寒性药玄参和热性药附子对大、小鼠心肌肥厚及神经内分泌因子的影响。方法：采用注射异丙肾上腺素及L甲状腺素两种方法分别制备小鼠和大鼠心肌肥厚模型。随机分为5组：正常组、模型组、美托洛尔组（或卡托普利组）、玄参组和附子组。灌胃给药干预7～9d，称体质量后取血和心脏备用。称心脏质量测定左心室及全心质量指数；放射免疫法测定血浆环磷酸腺苷（cyclicadenosinemonophosphate，cAMP）和心肌组织血管紧张素Ⅱ（angiotens

2、inⅡ,AngⅡ）的水平；病理切片HE染色观察心肌细胞横断面面积大小。结果：寒性药玄参能显著改善心脏质量指数，降低cAMP、AngⅡ的含量，明显缩小心肌细胞横断面面积。而热性药附子则无此作用。结论：寒性药玄参能通过抑制心肌肥厚大鼠交感神经系统活性，降低AngⅡ水平而抑制心肌肥厚。而热性药附子抗心肌肥厚及抑制神经内分泌因子分泌的作用不显著。【关键词】玄参;附子;心肌病,肥厚性;对比研究;大鼠;小鼠基金项目:国家自然科学基金资助项目（No.30572379）　　GuWL,ChenCX,WangY.J

3、ChinIntegrMed/ZhongXiYiJieHeXueBao.2008;6(4):376380.　　ReceivedSeptember6,2007;publishedonlineApril15,2008.　　Freefulltext(PDF)isavailableatwww.jcimjournal.com.　　Indexed/abstractedinandfulltextlinkoutatPubMed.　　ForwardlinkingandreferencelinkingviaCro

4、ssRef.DOI:10.3736/jcim20080410OpenAccess　　ComparativestudyofScrophulariaeandAconiteininhibitingmyocardialhypertrophyinratsandmice　　WeiliangGU,ChangxunCHEN,YingWANG　　DepartmentofPharmacology,ShanghaiUniversityofTraditionalChineseMedicine,Shanghai201

5、203,China　　Objective:ToexploretheeffectsofScrophulariaeofcoldnatureandAconiteofhotnatureonmyocardialhypertrophyandneuroendocrinefactorsinratsandmice.Methods:Amousemodelofmyocardialhypertrophywasestablishedbyhypodermicinjectionofisoproterenol.8Sixtymy

6、ocardialhypertrophymicewererandomlydividedintofivegroups:normalcontrolgroup,untreatedgroup,metoprololtreatedgroup,ScrophulariaetreatedgroupandAconitetreatedgroup.AratmodelofmyocardialhypertrophywasestablishedbyperitonealinjectionofLthyroxin.Fifty

7、ratswererandomlydividedintofivegroups:normalcontrolgroup,untreatedgroup,captopriltreatedgroup,ScrophulariaetreatedgroupandAconitetreatedgroup.After79daysoftreatmentwithintragastricadministrationofthecorrespondingdrugs,theeffectsofScrophulariaeand

8、Aconiteonleftventricularweightindex(LVWI)andheartweightindex(HWI)weredetermined.Theconcentrationsofcyclicadenosinemonophosphate(cAMP)inplasmaandangiotensinⅡ(AngⅡ)inmyocardiumweredetectedthroughradioimmunitymethod.Cardiocytecrosssectionareawasdeterm