资源描述：
《900mhz微波辐射对γ射线致小鼠造血系统损伤的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、低强度微波对γ射线辐射的防护作用及其可能机制吴兴宇苏州大学放射医学与公共卫生学院苏州215123【摘要】目的研究低强度微波对受照射小鼠的辐射防护作用及其可能机制。方法96只雄性昆明小鼠随机分为对照组、微波照射组（120μW/cm2，900MHz，SAR=0.4W/Kg，简称微波组）、γ射线照射组（5Gy，简称电离组）及微波与γ射线复合照射组（120μW/cm2+5Gy，简称复合组）。微波组与复合组小鼠先接受功率密度为120μW/cm2的微波照射，1h/d，持续14d，第15d给予电离组和复合组5.0Gy6
2、0Co-γ射线一次性全身照射。于照射后3d、6d、9d及12d处死动物，固定胸骨及脾脏，作病理观察，流式细胞仪检测受照射小鼠的脾细胞凋亡率，同时测定血清中抗氧化酶和脂质过氧化物水平。结果骨髓病理切片显示，单独γ射线与复合照射均可导致骨髓组织经历典型的凋亡坏死、空虚、再生修复和恢复4个阶段的病理改变，但复合组的病变轻于电离组，恢复也更快；脾损伤病变过程与骨髓造血组织基本相似，复合组病变轻于电离组；流式细胞仪结果显示，照后第6d、第9d复合组脾细胞凋亡率显著低于电离组；氧化损伤检测表明复合组血清SOD水平较电
3、离组升高，脂质过氧化水平显著降低。结论特定强度的微波辐射具有一定的辐射防护作用，其作用机制可能与抑制辐射诱导的造血细胞凋亡和抗氧化损伤作用有关。【关键词】低强度；微波；辐射防护StudyofradiationprotectionandpossiblemechanismsforlowintensitymicrowaveWuXing-yuSchoolofRadiationMedicineandPublicHealth,SowchowUniversity,Suzhou215123,China[Abstract]
4、ObjectiveToinvestigateradiationprotectionandpossiblemechanismsoflowintensitymicrowaveonγ-rayexposedmice.Methods96healthyKunmingmicewererandomlydividedintothefollowingfourgroups:normalcontrol,microwave(120μW/cm2,900MHz),γ-rayirradiation(5Gy),combinedexposu
5、reofmicrowaveandγ-ray(120μW/cm2+5Gy).Themicrowavegroupandcombinedgroupwereexposedto120μW/cm2microwavefirstly,1h/d,sustained14days,thentheionizationandcombinedgroupwereexposedto5.0Gy60Co-γ-rayirradiationatthe15thday.Animalsweresacrificedatthethird，6th，9tha
6、nd12thdayafterirradiation.Thesternumandspleenparaffinsectionwereproduced,andthehistologicalchangeswereobserved.Apoptosisrateofmicespleniccellsineachgroupwasexaminedbyflowcytometry,andserumconcentrationofantioxidaseandlipidperoxidewasdetectedatthesametime.
7、ResultsBonemarrowwasobviouslyinjuredeitherbyradiationormicrowaveexposure,characterizedbyundergoingfour-phaselesions,namelyapoptosis-necrosis,void,regenerationandrecoveryphase.Comparedtoγ-raygroup,thepathologicalchangesincombinedgroupwereslighterandthereco
8、verywasquicker.Thepathologicalinjuriesofspleenweresimilarlytothatofbonemarrow.Injuriesinthecombinedgroupwereslighterthanγ-raygroup.Itisalsoshowedthatapoptosisrateofspleniccellsincombinedgroupwassignificantlyloweratt
