肿瘤的生物治疗ppt课件

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1、癌症的生物治疗癌症的传统治疗方法：手术治疗放射治疗化学治疗癌症的生物治疗方法：基因治疗免疫治疗细胞治疗抗体治疗分子靶向治疗癌症治疗现状ProgressinGeneTherapyofLiverCancersMay4,2007GeneticMaterialGeneTransfer(vectors)BiologicalEffectTargetCellGeneTherapyMultistagemodelofcarcinogenesisChemicalRadiationVirusNormalcellPreneoplasticlesionMalignant

2、tumorMetastasisPositiveOncogenesGrowthfactorsProteasesAdhesionreceptorsMotilitycytokinesNegativeTumorsuppressorgenesGrowthinhibitorsMetastasissuppressorgenesGenetherapystrategiesfortreatmentoflivertumorsGeneTherapyforHCC1.GeneReplacementWild-typep532.AntisenseStrategiesAntis

3、enseRNA,Ribozymes,siRNA,shRNA,miRNAagainstOncogenesandGrowthFactor(ras,myc,BCL-2,IGF-2,TGF-a)3.Anti-angiogenictherapyTumorSuppressorGenesGenesLocationCancersp5317p13SmallcelllungcancerColorectalcarcinomaHepatocellularcarcinomabreastcancerLeukemiaRB113q14RetinoblastomaSmallce

4、lllungcancerOsteosarcomaBreastcancerWT111p13Wilm’stumorNephroblastomaDCC18q21ColerectalcarcinomaNF117qNeurofibromasAPC5q21ColorectalcarcinomaAntitumoralmechanismsinducedbytumorsuppressorgenesNat.Bio.Tech.2004AntitumoralmechanismsinducedbyinhibitionofoncogenesMechanismsofinhi

5、bitionofoncogenesbyshRNAandmiRNACo-expressionofshRNAandtherapeuticgenesfortherapyofcancerMechanismsofDrugSensitivitybyHSV-tkGanciclovir(GCV)MonophosphateGCVTriphosphateGCVDNADNApolymeraseChainterminationCelldeathreplicationCMV-HSV-tk-pAAdCMVtkBystandereffectinducedbysuicideg

6、eneIntroductionofHSV-tkintumorcellsGanciclovirEffect1:DeathoftranducedcellsEffect2:DeathofnearbycellsCancancerbetreatedbytargetingMETsignaltransductionpathwayInvolvementofcytokinesandgrowthfactorsinpathogenesisofcancerHepatocytegrowthfactor(HGF)MET(c-met)Hepatocytegrowthfact

7、or(HGF)MET(c-met)ExtracellularenvironmentSpecificproteaseHGFMET(c-met)ThesignalingpathwaysforMET-dependentinvasivegrowthExpressionofc-metinHCCUekiTHepatology.1997Mar;25(3):619-23.HGF-dependentinvasivegrowthandangiogenesisMEToverexpressionOverexpressedreceptors,locatedatthece

8、llsurface,undergospontaneousdimerizationandsubsequentactivationMETgeneampli