植物源蛇床子素生物农药创制与使用技术_（nxpowerlite）课件

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1、植物源杀菌剂蛇床子素创制及作用机制、使用技术研究石志琦范永坚田间种植的蛇床子植株Cnidiummonnieri(L.)Cusson有效成分：蛇床子素（7-methoxy-(3-methyl-2-butenyl)coumarin从伞型花科植物蛇床子提取1%蛇床子素EW,防治瓜类、草莓等白粉病特效，每亩用量有效成分仅2g，防效达90~98%。不产生抗药性！1%蛇床子·8000IU/µLB·tSE,防治甘蓝、花椰菜小菜蛾、菜青虫等鳞翅目害虫特效，防效达90~95%，且能杀夜蛾卵。产品获得有机认证1%蛇床子素EW40µg/ml25%粉锈灵WP1%蛇床子素E

2、W防治南瓜白粉病效果25%粉锈灵后五天25%triadimefon,in5d1%蛇床子素后五天1%osthol，in5d清水对照CKEffectofostholonthemorphologyofS.fuliginea.(a)BlankcontrolofSEM;(b)SEMofS.fuliginea96hafterthesprayof50µg/mLofosthol;(c)blankcontrolofTEM;(d)TEMofS.fuliginea96hafterthesprayof50µg/mLofosthol.A,B:Singledetectedle

3、avesfromplant6daysafterinoculatedwithS.fuligineaconidiospores.A,100μg·mL-1ostholpretreatedleaves;B,Solutioncontrol;C,D:AnilinebluestainingcoloniesofS.fuligineadetectedleavesfromplant6daysafterinoculated.C,ThemyceliumgrowingofS.fuligineaaftertreatedwith100μg/mlosthol,D,Solution

4、control.1dpi2dpi3dpi4dpi5dpiBlankSolvent50ppm100ppmEffectofostholonthelifecycleofSphaerothecafuligineaconidiaonpumpkinleavesOsthol不能延迟分生孢子的侵染循环，但抑制了菌丝的生长，菌落变得稀薄，产孢量也明显下降。EffectofostholonsporesgerminationonpumpkinleafdiscValueswithletterincommonwithinacolumnarenotstatisticallys

5、ignificant(p<0.05),LSDtest.叶盘法100µg∙mL-1Osthol对白粉菌孢子萌发抑制与对照相比差异显著，溶剂对萌发的影响很小treatments空白3h12h24h36h48h7.58%a35.26%a43.21%a55.81%a57.14%asolvent4.77%a31.28%a33.27%b52.29%a52.69%aosthol0%b3.60%b3.66%c3.72%b3.89%bEffectofostholonsporeadhesiontopumpkinleaves24h48h72hNumberofconidi

6、aandgerminationrateNumberofconidiaandgerminationrateNumberofconidiaandgerminationrateWithoutrinsebydropscontrolTotalconidia99.55±34.130.5198±0.09a61.25±11.540.5846±0.11a60.1±13.60.6764±0.13aGerminatedcondia50.45±16.5835.4±8.2040.1±9.90SolutionTotalconidia97.25±35.650.5186±0.12

7、a66.4±12.270.6246±0.10a60.8±12.440.6666±0.11aGerminatedcondia49±17.2141.1±9.1840.35±10.3ostholTotalconidia82.4±42.780.3457±0.11b64.65±43.670.2701±0.13b57.5±22.560.1842±0.08bGerminatedcondia27.95±17.2316.8±14.1710.2±6.30RinsedbydropscontrolTotalconidia86.40.528862.350.543863.65

8、0.6836Germinatedcondia44.133.8543.2SolutionTotalconidia99.70.