牛磺酸对小鼠肠推进及大鼠离体回肠平滑肌运动的影响

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1、第14卷4期天津医科大学学报Vo1．14．No．42008年l2月jouRNALOFTIANJINMEDICALUNIVERSITYDec．2oo8牛磺酸对小鼠肠推进及大鼠离体回肠平滑肌运动的影响刘妍妍。崔志清(天津医科大学药理学教研室，天津300070)[摘要]目的：观察不同浓度的牛磺酸(Tau)对小鼠在体小肠推进运动及大鼠离体回肠平滑肌收缩运动的影响并初步探讨其作用机制。方法：小鼠灌胃给药后测定小肠中阿拉伯胶活性炭粉混合物的推进距离并计算小肠炭末推进比；采用离体器官实验法。以回肠平滑肌的收缩幅度及收缩频率为指标，观察在

2、含钙及无钙营养液中牛磺酸对大鼠离体回肠平滑肌的影响．同时观察硝苯地平对牛磺酸的拮抗作用。结果：低、中剂量牛磺酸可增强小鼠小肠运动功能(P

3、用。[关键词]牛磺酸；小肠推进；离体回肠；平滑肌；钙离子[中图分类号]R965[文献标识码]A[文章编号]1006—8147(2008)o4—0441—04EfectsoftaurineonsmallintestinalpropulsioninmiceandisolatedileumsmoothmuscleofratsLIUYan-yan，CUIZhi-qing(DepartmentofPharmacology，TianjinMedicalUniversity，Tianjin300070，China)ABSTRACTObj

4、ective：Toobservetheeffectsandmechanismsofdifferentconcentrationoftaurine(Tau)onsmallintestinalpropulsioninmiceandcontractionactivityoftheisolatedileumsmoothmuscleofrats．Methods：Themicewerekilledaftergivendrugsandcarbonpower,smallintestinalcarbonpowderpropul—sionra

5、tewasobserved；moreover．theisolatedileumsmoothmusclefromratswasprepared。andcontractionamplitudeandfrequencyofthesmoothmuscleweremeasuredinthenormalandcalcium-freeKrebs——Hense．1eitsolutionsandtheantagonisteffectofnifedipineontaurinewasobserved．Results：Comparedwithth

6、econtrolgroup，boththelowandthemiddledosageoftaurinecouldenhancesmallintestinalpropulsioninmicerespectively(P<0．05andP<0．01)，whilethehighdosageoftaurinehadtheoppositeeffect(P

7、rinecouldenhancethecontractionamplituderespectively(P<0．01)，butnotchangethe~equencyofisolatedileumsmoothmuscleandtheeffectofthemiddledosagegroupwasmoreconspicuousthanthatofthelowdosagegroup(P<0．05)；whilethehighdosageoftaurineproducedtheoppositeresult(P

8、eexcitedeffectofthemiddledosageoftaurineontheileumsmoothmusclewascompletelyblockedbythecalciumchannelblocker,nifedipine)<0．o1)．Conclusion：Taurinecanenha