壳聚糖乙酰化甲壳素材料与许旺细胞的体外相容性

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1、万方数据壳聚糖乙酰化甲壳素材料与许旺细胞的体外相容性冰水冰术★◆陈颖’，任艳玲2，李奕1。林巍巍1，杨宁民2，王晓冬1Biocompatibilityofchitinacety’iatedfromchitosanwithSchwanncells伽vitroChenYin91，RenYah一¨n92，L．Yil，LinWei—weil，YangYu-rnin2，WangXiao-don91Abstract1DepartmentofHistologyandEmbryology．MedicalCollegeofNantongUniversity,Nantong226001．JiangsuPro

2、vince，China；‘KeyLaboratoryofNeuroregenerationofJiangsuProvince．Nantong226001。JiangsuProvince．ChinaChenYing★．Studyingformaster’sdegree．DepartmentofHistologyandEmbryology，MedicalCollegeofNantongUniversity，Nanmng22600l，JiangsuProvince，China06160034@rltn．o血L∞Corccspondenccto：WangXiao—dong。ProfeSSOr,T

3、utorofdoctor,DepamaentofHistologyandEmbryology,MedicalCollegeofNantungUniversity,Namong226001．JiangsuProvince，Chinawxdzw@nm．edu．enSupportedby：theNationalNaturalScienceFoundationOfc11inn,No．30770585*；theNaturaIScienceFoundationofJiangsuProvince．No．07KJA310250：SocialDevelopmentandScientificP1allofN

4、antong，No，S50038’：NewCenturyExcellent1柚entProgramoftheMimstryofEducatioll．No．NCEW埘66+BACKGRoUND：Naturalchitinispoordissoluble．thushindersthefurtherprocessing．Chitinpreparedbytraditionaltechnologyholdsmanyimpureproteinsthatinduceinflammation．OBJECTIVE：ToevaluatethebiocompatibilitybetweenSchwanncel

5、lsandchitinthatacetylatedfromhighpuritychitosaninvitro．DESIGN。TIM[EANIlSEllrING：Thisexperimentwasdesigned勰comparativeobservation．andcarriedoutinDepartmentofHistologyandEmbryology，MedicalCollegeofNantongUniversityandKeyLaboratoryofNeuroregenerationofJiangsuProvincefromSeptember2006toOctober2007．MA

6、TERIALS：NeonatalSDrats，l一2daysoldandSPFgrade，wei'eusedasdonors，thesciaticnerveandbrachialplexusnervewereharvestedtocultureSchwanncells；Chitosanmembranewasself-madeintoadeacetylationdegreeof92．5％．M[ETHoDS：chitosanmembrane(degreeofdeacetylation：92．5％1wasimmersedin5％aceticanhydrideofmethanolsolution

7、．TheSchwanncellswereco—culturedwithchitosanmembraneandchitinmembrane．respectively．MAINoUTCOMEMEASURES：Subsequenttotheco-culture．SchwanncellswereidentifiedbyimmunocytochemicalS．100staining，themorphologyofSchwanncellswas