樟巢螟雌蛾性信息素主要组分鉴定及诱蛾活性（英文）

《樟巢螟雌蛾性信息素主要组分鉴定及诱蛾活性（英文）》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、2016bioassaysanditseficacyinprelimi-theextractswereidentifiedbycompar—E11—16：AIdwassynthesizedfrOmnaryfieldevaluation．ingtheirretentiontimesandmassE11-16：Ac．Eachsynthesizedcom．spectrawithsyntheticstandards．poundwaspurifiedusingsilicageIflashMaterialsandMethodsSynthe·ticstandards(pheromonechromato

2、graphy，resultingint>97％Insec~LateinstarIarvaewerecomponentsandcamphortreeleafpurity(asconfirmedbygaschromatog-collectedfrOmcamphortreesinNing—volatiles)Thesyntheticpheromoneraphy)，transferredtocleanglassvialsbobetweenJulyandSeptemberincomponentsE11-16：Ac．Z9—16：AIdandstoredat-20℃priortouse．Plant20

3、11and2012．TheselarvaewereandZ11-16：AcwerepurchasedfrOmvolatilesassociatedwithcamphorwerereareduntiIeclosiononsmalIcamphorBedoukianResearchInc．(Danbuw，selectedfr0mthework．ThesewerebranchesincagesinthecontrolledConn．．USA)．1ncontrast．Z11—16：Aldcis-3一HexenyIAcetate(Z3．6：Ac)，(L)．room(14L：10D，27℃)．Newl

4、yem．andZ11—16：OHweresynthesizedLinalool，(1S)-(一)一a-pinene，(1S)一(·)-13-ergedmothswerecollectedtwicedai．frOmZ11-16：Acbyhydrolyzationandpinene，geraniol，benzaldehydeand1y．Thesexeswereseparated，main．oxidizationwithpyridiniumchlorochro．phenylacetaldehydeandwerepur-tainedinscreencagesf30cmx20cmxmate(PCC

5、)．UsingthesameprocesschasedfromSigma—Aldrich(St．Louis。盘15cm1andprovidedwithadilutesug．580000arsolutionad／／b．oncottondisks．PheromoneextractionPhero．moneglandswereexcisedfr0m2一tO60o003-day-oldvirginfemalemoths，ap．proximately5—6hintothescotophase．40000Theabdomenofeachfemalem0thwasgentlysqueezedtoeve

6、dthe20000gland，grippingtheabdomenjustante—riortotheglandwithforceps，andslic．ingtheglandofwitharazorblade1920212224suchthattheglandremainedinflatedP,~tenti∞t[meintheforceps．Theglandsfr0mbe．Fig．1GC-MSanalysisofcrudepheromoneextractsfromfemaleOdhagaachatinamothstweentwoandfivemothswereeach喑∞gds0。v目p

7、lacedfor30secinca．200uIofdou．LLbledistilledhexaneinaglassviaItoextractthepheromone．Atotalof30．1．separateextractswereproducedjnthisway．Eachextractwastransferred量旺ntothepointedglassviaImadefrOm曲glasscapillaries，concentra