新型海藻酸盐水凝胶真皮支架材料的制备及其表征

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1、万方数据中国组织T程研究与l临床康复多享，2眷第4，雳2008—10—0r7出版JournalofClinicalRehabilitativelissueEngineeringResearchOctober7'2008V01．12．No．41炙】乏震基础实验新型海藻酸盐水凝胶真皮支架材料的制备及其表征☆朱家源，王琴梅，朱斌，唐冰，蔡浩Preparationandexosyndromeofsodiumalginatehydrogeiasadermaltissueengineeringscaffold

2、ZhuJia-yuan，WangQin-mei，ZhuBin，TangBing，CaiHaoAbstractBACKGROUND：Sodiumalginatehydrogelsareidenticalwiththeproteoglycan，adermalmatrixcomponent，regardingthestructure．Itisanidealmaterialforfibroblastculture．However，Sodiumalginatehydrogelsarepreparedtaki

3、ngCaCl2ascrosslinkingagent，whichisadefectivemethod．OBJECTIVE：Taking1-ethyl一3一f3·dimethylaminopropyl)一carbodiimide舔thecatalystandethylenediamineascrosslinkingagent,thesodiumalginalzhydrogelsarepreparedasanewkindoftissueengineeringscaffold，itsphysicalch

4、emistryperformanceisanalyzed，anditsfeasibilitytobedermissubstituteasthetissueengineeringscaffoldisapproached．DESIGN，TIMEANDSETTING：Theexperiment，amorphologyobservationandself-controlledstudy，wasperformedinDepartmentofOrthopedics．theFirstAffiliatedHosp

5、italofSunYat—senUniversityfromOctober2005toOctober2006．MATERIALS：Thel—ethyl一3一(3-dimethylaminopropyl)一carbodiimide，ethylenediamineandsodiumalginate廿'erepurchasedfromsigmaCompanyMETHODS：Using1-ethyl一3一f3·dimethylaminopropyl)-carbodiimideasacatalystande

6、thylenediamineasacrosslinkingagent,sodiumalginatehydrogelscaffoldswerepreparedwithcovalentcross—linking。freezinganddryingmethod．ConventionalsamplepreparedusingCaCl2ascrosslinkingagentwastakenascontrols．MAINoUTCoMEMEASURES：Theidealresponseconcentration

7、ofsodiumalginatewasobserved；themicrostructureofalginatescaffoldfortissueengineeringwasdetectedusingscanningelectronmicroscope；thewatercontentandporosityofalginateseaffoIdweredeterminedusingpushingliquidaside；thedegradation／nvitrowasalsoobserved．RESULT

8、S：111e．sodiumalginatehydrogelsfortissueengineeringdermiswerepreparedSuccessfully．Idealreactionthicknesswas2％。porositywas(85．55±0．98)％，thewatercontentwas(95．32±1．24)％，whichwerehigherthanthoseofconlrolmaterials：T11esodiumalginatehydrogellostf12．