新型胶原真皮支架材料的制备与性能表征

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1、■●山东大学硕士学位论文PREnU王ATIoNANDPRoPERTIESoFNoVELCoLLAGENDERMALSCAFFoLDMATER工ALABSTRACTHumanskinisasoftoutercoveringofthebody,whichplaysakeyroleinprotectingthebodyagainstdamagefromtheenvironments．Slightlydamagedskinwillgethealedbasedontheregenerativeabilityofhumanbody,while

2、anylossoffull—thicknessskinofmorethan4cmindiameterwillnothealwellwithoutsurgicalintervention．Allograffsandautograftshavebeenemployedforwoundhealingoflargedermaldefects；however,constraintsonobtainablequantities，antigenicityandriskofdiseasetransmissionlimittheiruse．Ther

3、efore，manystudiesareturningtowardtheskintissueengineeringapproachtopromoteskintissueregeneration．Skintissueengineeringistheemergingdisciplineofdesignandconstructionofsparepartsforhumanskintorestorefunctionbasedonprinciplesofmoleculardevelopmentalbiologyandmorphogenesi

4、sgovernedbybioengineering．Dermalscaffoldmaterialisoneofthethreefactorsofskintissueengineering．Followingtheguidanceoftheperformancestandardsofdermalscaffoldmaterialsinskintissueengineering，anovelcollagendermalscaffoldmaterialwithnativethree··dimensionalstructureandidea

5、lmicrostructurewaspreparedusingalow·-costandsimplepreparationtechnology．Theparametersofthepreparationwereoptimizedandthepropertiesofthescaffoldmaterialwerecharacterized．(1)ThepreparationtechnologywasdesignedbasedonthetraditionalmethodsofADMpreparationandthematurepretr

6、eatmentsofleathertechnologyusingporcineskinastherawmaterialwhichwasstructurallyandimmunologicallysimilartohumanskin．Thepreparativescaffoldmaterialcontainednoporcinecellsandboreidealmicrostructurebasedonretainingnativecollagenthree—dimensionalstructure．Pretreatmentsrem

7、ovedfinefair,sweatgland，subcutaneoustissueandSOon．Thecombinedtreatmentswithenzyme，alkali，sodiumdodecylsulfonateandsodiumchloridesolutioncontributedtothefinaleasyoperation，lowcostandidealpropertiesofthescaffold．Afteroptimizationsbyadjustingconcentrationsandtimeand3山东大学

8、硕士学位论文analyzingmicrostructure，mechanicalproperties，porosities，aminoacidcompositionsandSOon，thefinaltechnologicalparameterswe