转化生长因子β1胰岛素样生长因子1与髓核细胞诱导脂肪间充质干细胞向髓核样软骨细胞分化的比较

《转化生长因子β1胰岛素样生长因子1与髓核细胞诱导脂肪间充质干细胞向髓核样软骨细胞分化的比较》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、万方数据中国组织工程研究与临床康复多争似誊劈23婀2010—06—04出版JoumalofClinicalRehabilitativeTissueEngineeringResearchJune4J2010V01．14,No．23转化生长因子131／胰岛素样生长因子1与髓核细胞诱导脂肪间充质干细胞向髓核样软骨细胞分化的比较术★刘连江他，李放2，文天用1Transforminggrowthfactor-beta1／insulin-likegrowthfactor-1versusnucleuspulposuscellsininduci

2、ngthedifferentiationofadipose-derivedmesenchymalstemcellsintonucleuspulposus·likecellsUuLian-jian91上，LiFan92。Wennan·yon91Abstract8ACKGROUND：Previousstudieshaveaddressedadipose-derivedmesenchymalstemcellsfADSCs)culturewayformonolayerculture；inductionwaywasmainlycytoki

3、nemethod．andunderintervertebraldiscsmicro-environment．celIInteractionsalsodeservedfurtherstudyinitsthree-dimensionaIenvironment．inadditiontotheeffectsoncelIcytokines．OBJECTIVE：ToobservedifferenceslndifferentiationofADSCsintoNPCsundertwojnducedconditions：nucleuspulpos

4、uscells(NPCs)andtransforminggrowthfactor131(TGF-111)／insulin-likegrowthfactor(IGF-1)invitro．METHODS：RabbitADSCsmonolayercultureandNPCswereculturedrespectively。3generationsofADSCswerequalified：5xl0rcelIsweremadeintomicrospheresandplacedinTranswellcuttureplatesforcultu

5、reunderTGF．13111GF一1induction．CelIshapechangeswereobservedbefore，7，14daysafterinduction．Reversetranscription-polymerasechainreaction(RT·PCR)wasusedtodeterminelevelsoftypeIIcollagenandproteoglycan．RESULTSANDCONCLUSlON：At7and14days，nosignificantdifferencewasdetectedins

6、izeandshapeofADSCsmicrospheresinbothgroups．RT-PCRdetectionresultsdisplayedthattypeIIcollagenandproteoglycanmRNAexpressionwasdeterminedin7一dayTGF-BI／IGF-1groupandNPCs-inducedgroup．butexpressionwasstrongerintheTGF—DI／IGF-1group．Type¨collagenandproteoglycanmRNAexpressio

7、nwasobyiouslyincreasedin14一daVNPCs-inducedgroup。superiortoTGF-IBI／IGF-1group．Resultshavesuggestedthat／nvitroNPCsandTGF一131／IGF-1IeadtotheADSCs．whichhadpromotingeffectsonNPCsdifferentiation．TGF一131andIGF-1arenormaINPCs-secretedimportantfactors．Thepromotingeffectsonpro

8、liferationandd讦femntiationexist．withtheexceptionofcytokineinductioneftect，indifferentiationofADSCsintoNPCs．Ljuu，uF，WenTY．Transformi