新式分层共培养环境下人骨髓间充质干细胞诱导髓核细胞的分化

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1、万方数据中国组织工程研究与临床康复第。74誊第7力咿2010—01一01出版JournalofClinicalRehabilitativeTissueEng加eeringResearchJanuary7，2010VoL14，No．7新式分层共培养环境下人骨髓间充质干细胞诱导髓核细胞的分化★李全修1，陈伯华1，蔡月艳2Differentiationofhumanbonemarrowmesenchymalstemcells-inducednucleuspulposuscellsundernew-stylelayercocultureLiQuan-xiul，Che

2、nBo-hual，CaiYue．yan2AbstractBACKGROUND：TherearesomeIimitationsininduceddifferentiationofbone-marrow-derivedmesenchymalstemcalls(hBMSC{andtransplantationofnucleuspulposuscellsfNPCs)．InhibitionofautologousNPCsdegenerationisexpectedlobecomeaneffectivewayfortreatmentofdiscdegenerationi

3、nthefuture．OBJECTIVE：ToestablishalayeredcoculturemodeIofhBMSCsandNPCs／n谛ro，andtoobservetheeffectofhBMSCsonthedifferentiationofNPCs．METHODS：ThefourthpassageofhBMSCswasdividedinto2groups．NPCsinthenew-styleIayerculturegroupwereincubatedinTranswelIcabinwithoutarnl。0．4．Ummembranewell；th

4、eproportiontoBMSCswas1：1．NPCsinthetraditionaIculturegroupwereincubatedinTranswelIcabinwitharnl．NPCculturegroupwasset．Eachgroupwasincubatedfor7days．CollagenITwasdetectedbyimmunoh．stochemicaImethod．Aggrecanexpressionwasdetectedbyr。S】-sulfateuptake．RESULTSANDCONCLUSION：ComparedwithNPC

5、culturegroup．collagenIIandaggrecanexpressionofNPCswereupregulatedmostevidenUyjnnew-stylecultureandtraditionalculturegroups(P<0．05，P<0．01)．Theincreasedrangeofnew-styleculturegroupwasgreaterthanthetraditionalculturegroupfP

6、ificantlyincreaseNPCproIiferationandspecificsubstancaexpression．Thesurroundingofnew-styleculturesurpassedthetraditionaIculture．LiQX，ChenBH。Caiyy'．Differentiationofhumanbonemarrowmesenchymalstemcells—inducednucleuspulposuscellsundernew-stylelayercoculture．ZhongguoZuzhiGongchengYanji

7、uyuLinchuangKangfu．2010；14(1)：53-56．【http：／／www．crter．cnht【p：flen．zglckf．com】摘要背景：诱导分化的骨髓间充质干细胞或髓核细胞移植都存在一定的局限性，抑制自体髓核细胞的退行性变有望成为未来治疗椎间盘退变的有效方法。目的：通过建立骨髓间充质干细胞和髓核细胞分层共培养模型，观察骨髓间充质干细胞对髓核细胞分化的影响。方法：取传至第4代的骨髓间充质干细胞，分为2组：新式分层培养组将髓核细胞接种于去掉挂臂的Transwell小室，膜孔0．4um，其与骨髓间充质干细胞比例为1：1；传统分层培养组同

8、法将髓核细胞接种于带有挂臂的Transwell小室。