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ID:18165327
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时间:2018-09-14
《携带人胰岛素样生长因子ⅰ基因的重组腺病毒体外转导培养关节软骨细胞》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、万方数据中国组织工程研究与临床康复第,2卷笫“勇咿2008—06一lO出版JournalofClinicalRehabilitativeTissueEnginee—ngResearchJune10,2008VoLl2,No.24携带人胰岛素样生长因子I基因的重组腺病毒体外转导培养关节软骨细胞江海良,夏青,魏振,黄爽,贾静Cultureofarticularchondrocytesthroughtransductionofadenovirus—humaninsulinlikegrowthfactor-Itransgene/nvitro.JiangHal-liang,XiaQing。Wei
2、Zhen,HuangShuang,JiaJingAbstractAIM:Insulinlikegrowthfactor-Iisalwaysregardedasoneofthekeygrowthfactorsincartilagegrowthandstability.ItcanpromotetypeIIcollagenandproteoglycanproductionbychondrocyte.Tllisstudyinvestigatedtheproductionofthesubstratecomponentofcartilagebyla'ansductionwithadenovirus
3、—humaninsulinlikegrowthfactor-I(Ad·hlGF-I)transgeneconstruct.METHODS:111eexperimentwasperformedattheLaboratoryofOrthopedics.FourthHospitalofDalianMedicalUniversityfromJanuary2005tOApril2007.ChondrocytesWereisolatedfrom3-week—oldrabbitkneeandfemoralheadandcultured.ThefirstpassagechondrocytesWerea
4、ssignedtotransductedgroupandnon—transductedgroupaftertheyweretransductedbyAd—hlGF—Iof500multiplicityofinfection.Thefirst.third.fifthandseventhpassagechondrocytes仃ansductedandnon—transductedwereassayedbyinvertedlightmicroscopytoobservetheirbiologicalcharacteristicchanges.ELISA,alkalinehydrolyseds
5、ampleassayandalicianbluetestwereperformedtoidentifytheexpressionandbiosynthesisofhydroxyproline,glycosaminoglycans(GAG)andIGF-Iprotein.RESULTS:Themicroscopicobservationshowedchondrocytesinthetransductedgroupproliferatedstably,andtriangle,andspindlecellsWerepredominantintheseventhpassage,accompan
6、iedbyfewlong·spindleandrootcells.Inaddition,thecellswereofclearappearanceandlargelucency.However,inthenon·transductedgroup,long—spindlecellswereobservedinthefourthpassage,andalmostallcellsexhibitedlong—spindleshapeinthefifthpassage.Wimculturetimeandpassageprolonging.theconcentrationofIGF-J.hydro
7、xyprolineandGAGintransductedchondrucytesculturemediumwassignificantlyhigherthanthes锄epassageceHsinnon—transductedgroup(P<0.05).CONCLUSIoN:Theresultssuggest山atchondrocytestransductedwithAd—hIGF-IcouldproduceendogenousIGF-Iand
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