大豆异黄酮对氧化损伤血管内皮细胞的抗氧化作用

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1、IssNI67I一5926CN2I—I470／R咖．础c帅删眦c帅刘英华，等大豆异黄酮对氧化损伤血管内皮细胞的抗氧化作用171Cultureandevaluationofendothelialcells：Aneranabiosishefrozencalculatedwiththeincomeabsorhancecombinedwitlltllepr0teinicendothelialcellswasinoculatedintllecellculturalboardwitllEaconcentrationinendochylema．gIe’scuIturem

2、ediummodiedbyDulbecco(containing100g／LStatisticalanalysis：SPSS10．0sofharepaekagew_asusedtope卜fetalbovinesemm1andthenculturedintheincubatorof37℃，f0mIthedatapmcessingbytllefirstautllor．0ne—wayanalysisof50mL／LC02，950mL／Lairsaturatedhumid．ty．1'hecellsgrewtovancewasusedtomakecompson．fl

3、useintopykno．monoIayerandthentheculturenuidwassloppedtoberemoved．TheyweredjgestedwiththeequivalencemixturesofRESULTS2．5g／Ltrypsinand2g／Ledathamilandthensubcutur℃dwith1：2．hInuenoeofsoybeanis0aaVoneonmalondialdImydIc0ntent'rheceIIsgrowingtofluseintomonolayerwereusedintheexperi·actie

4、sofsuperode蜘uta!andgIu纽thio眦per0嫡凼Isement．intheendothelialcelIs0fo嫡davedamageable1)SolutionofvitaminEandsovbeanisonavone：Themixturesofab—Table1showedthattlledi珏lerencesofeach{mupallwereofsig．soIuteaIcohoIanddimethvIsulf0xidewer℃usedtodissolve．nificanceinthe3indexeswhethertheywerei

5、ncellularorextm—Dosagegr0uping：Theexperimentwasdividedinto6gmups：blankcellular<0．01)．Amongtllem，themal0ndialdehydecontentwascontmlgmup，oxidativedamagecontmlgmup(malondialdehydehighersignificantlyinoxidativedamagecontrDlgmupthanincontentwas1moI／L】，oxidativedamage+vitaminEcontmIgmup

6、blankcontrolgroup(P<0．01)，buttheactivtyofsuperoxidedismu·(vitaminEwas50mol／L)andoxidativedamage+soybeantaseorglutathioneperoxidasewaslowersignificantlyinoxidativeisonavone10，50，100mol／Lcontr0lgmup．damagecontrolgmupthaninblankcontrolgmup(P<0．01)．Vita—Resear_chmethod：TheceIIsofgmwin

7、gtofuseintothemonoIayerminEandsoybeanisonavonewereaddedinadvancetomakethewereg0ttosloppetoremovetheculturenuidandwashedf0r2ormalondialdehydecontentr℃duce(P<0．01)andtheactivityofsu—3timeswithbuflferphosphate．Thecellcultumlnuidwasrenewedper0xidedismutaseandglutatllionepemxidaseincre

8、ase<0．01)．andvitaminEandsovbeanis