5 km高空缺氧对小鼠肝脾及血清中Zn ，Cu和Fe的影响.doc

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1、5km高空缺氧对小鼠肝脾及血清中Zn，Cu和Fe的影响【摘要】　　目的：探讨5km高空缺氧和不缺氧对小鼠肝、脾及血清中Zn,Cu,Fe的影响.方法：高空缺氧试验：将雄性昆明种小鼠20只，随机分为实验组(5km高空缺氧30min组)和地面对照组，每组10只.将实验组小鼠置于低压舱内，以40m/s的速度上升到5km，停留30min后，以40m/s速度降至地面；地面对照组放置在另一低压舱中留在地面.连续7d后，抽取小鼠静脉血液并处死小鼠取肝、脾.高空不缺氧试验：分组及实验方法同上.舱内压力为54.02kPa，其中氧

2、分压为21.2kPa.舱内容积百分比为41.8%氧和58.2%氮的混合气体.用测氧仪监测舱内氧压，保持其肺泡氧分压与地面相同.采用火焰原子吸收法测定血清Zn，Cu和Fe的含量.结果：5km高空缺氧组与对照组相比较,小鼠肝中Cu和Fe含量均显著降低,差异有统计学意义(P<0.05)；脾中Fe的含量明显升高,差异有统计学意义(P<0.05)；两组血清中Zn，Cu和Fe含量均无明显变化,差异无统计学意义(P>0.05).5km高空不缺氧组与对照组相比较,小鼠肝、脾中Zn，Cu和Fe含量均降低,差异

3、具有统计学意义(P<0.05),血清中Zn，Cu和Fe含量均无明显变化,差异无统计学意义(P>0.05).结论：5km高空缺氧时对小鼠肝中Cu和Fe的合成产生影响；5km高空缺氧和不缺氧时小鼠血清中Zn，Cu和Fe的含量无明显变化，表明其含量可能与机体缺氧无关.【关键词】高海拔缺氧；肝；脾；血清；锌；铜；铁【Abstract】AIM:Toexploretheinfluenceof5kmaltitudehypoxiaandanoxiaonZn,Cu,Feinmouseliver,spleenands

4、erum.METHODS:Experiment1:20malemiceweredividedintoexperimentalandcontrolgrouprandomly(n=10).Theexperimentalgroupwasputintolowpressuremoduleandascendedto5kmaltitudeatthespeedof40m/sandstayedfor30min,thendescendedtothegroundatthesamespeed.Thecontrolonewasput

5、intoanotherlowpressuremoduleontheground,successivelyfor7d,andthenthemicewerekilledandlivers,spleensandseraweretaken.ThecontentsofZn,CuandFeweredeterminedbythemethodofflameatomabsorption.Experiment2:Thegroupingandmaneuverswerethesamewiththeaboveexperimentex

6、ceptthatthepressureinthemodulewaskept54.02kPaintotalpressureand21.2kPainpartialpressureandthemixedgascomprised41.8%oxygenand58.2%nitrogen.Thealveolaroxygenpartialpressurewasmonitoredbyoxygenanalyzerinmoduletokeepthesamewiththeoneatground.RESULTS:Inexperimen

7、t1,comparedwithcontrolgroup,thecontentsofCuandFeinliverweresignificantlydecreased(P<0.05)andtheFecontentinspleenwassignificantlyincreased(P<0.05)intheexperimentalgroup;thecontentsofZn,CuandFeinbothgroupswerenotsignificantlydifferent(P>0.05).Inexper

8、iment2,4comparedwithcontrolgroup,thecontentsofZn,CuandFeweredescendedsignificantlyinexperimentalgroup(P<0.05),whilethealterationsintheseraofexperimentalgroupwerenotsignificantstatistically(P>0.05