欢迎来到天天文库
浏览记录
ID:17458339
大小:34.50 KB
页数:5页
时间:2018-09-01
《原发性肝癌患者细胞因子水平的变化与转移和复发的关系.doc》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、原发性肝癌患者细胞因子水平的变化与转移和复发的关系作者:滕木俭王明海潘乐玉【摘要】目的:观察原发性肝癌患者细胞因子IL4、IFNγ、IL1β在肝癌组织中的表达,探讨细胞因子的变化、漂移与肝癌转移和复发的关系。方法:应用逆转录聚合酶链反应(RTPCR)检测并比较肝癌组和对照组细胞因子IFNγ(Th1)、IL4(Th2)、IL1β的水平。结果:34例肝细胞性肝癌患者与15例单纯肝硬化患者相比,肝癌组织中IFNγ(Th1)的表达强度、阳性表达频度较对照组明显降低,分别为0.92±0.36(18/34,53.9%)和1.63±0.64(12/15,80.0%)(P<0
2、.01)。IL4(Th2)在肝癌组织中的表达强度较对照组明显增强,分别为1.76±0.49和1.01±0.51(P<0.01)。IL1β在肝癌组织中和对照组的表达强度及频度分别为1.01±0.32(30/34,88.2%)和0.35±0.30(4/15,26.7%)(P<0.001)。3种细胞因子在肝癌组织中有5种不同表达方式。结论:原发性肝癌患者的抗肿瘤免疫存在严重缺陷,细胞因子间的平衡被打破,使已处于抑制状态的Th细胞免疫更趋恶化,致使肿瘤细胞容易发生肝内转移及术后复发。【关键词】肝肿瘤・白细胞介素1・白细胞介素4・
3、;干扰素Ⅱ型・肿瘤转移・肿瘤复发,局部Therelationshipbetweenimmunoregulatorycytokinesandintrahepaticmetastasisandrecurrenceinprimaryhepatocellularcarcinoma【ABSTRACT】Objective:Toobservetheexpressionofimmunoregulatorycytokinesincludinginterleukin4(IL4,Th2)、interferonγ(IFNγ,Th1)、interleukin
4、betal(IL1β)inthetissuesofhepatocellularcarcinoma(HCC),andtoinvestigatetherelationshipbetweenthechangesofimmunoregulatorycytokinesinpatientswithhepatocellularcarcinomaandintrahepaticmetastasisandrecurrence.Methods:Theimmunoregulatorycytokines:IFNγ(Th1)、IL4(Th2)、IL1βwereanalyzedbyRTPC
5、R.Results:34caseswithHCCwerecomparedwiththeoneswithlivercirrhosis,theexpressionratesanddensityofIFNγ(Th1)inthetissuesofhepatocellularcarcinomaweremuchlowerthanthatinthelivercirrhosis,theyare0.92±0.36(18/34,53.9%)and1.63±0.64(12/15,80.0%),respectively.Thereissignificantdifferencebetw
6、eenthem(P<0.01).TheexpressiondensityandlevelsofIL4(Th2)inthetissuesofhepatocellularcarcinomaincreasedclearly,comparedwiththecontrolgroup.Theyare1.76±0.49and1.01±0.51,respectively.Theirdifferenceshowedstatisticalsignificance(P<0.01).TheexpressionratesandlevelsofIL1βinthetissuesofhepa
7、tocellularcarcinomaandlivercirrhosiswere1.01±0.32(30/34,88.2%)and0.35±50.30(4/15,26.7%),respectively.Therewassignificantdifferencebetweenthem(P<0.001).Abovethreeimmunoregulatorycytokinesinpatientswithhepatocellularcarcinomashowedfivedifferentexpressionstyles.Conclusion:Thereissevere
8、deficiencyofimmuner
此文档下载收益归作者所有