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1、门静脉高压症大鼠阻断门静脉和下腔静脉后肾脏损伤的研究作者：吴凤东窦剑赵鑫任贵军邸恩昌【摘要】目的：探讨门静脉高压症大鼠阻断门静脉和下腔静脉1h后肾脏损伤的情况。方法：Wistar大鼠随机分为对照组、门脉高压症对照组和实验组。实验组先建立可复性门静脉高压症大鼠模型，3周后按阻断下腔静脉和肝门1h后不同的再灌注时间0h、6h、12h、24h、48h、72h和7d分为7组。于相应时间点查血清ALT、BIL、BUN、Cr水平，肾脏和肝脏组织光镜以及肾脏组织透射电镜检查。结果：实验组大鼠血BUN、Cr再灌注后6h开始升高，12~24h达高峰，此后渐下降，7
2、2h已基本恢复正常。肾脏损害主要以近曲肾小管上皮细胞为主，12h、24h损伤达到高峰，可见核固缩、核碎裂，但基底膜保留完好。48h开始出现修复现象。7d后明显修复。结论：门静脉高压症大鼠阻断门静脉和下腔静脉后，肾小管上皮细胞有明显损伤，但是损伤是可逆的。【关键词】高血压门静脉・腔静脉，下・肾・大鼠，WistarThestudyofrenalinjuryinportalhypertensiverataftertheocclusionofportalveinandinferiorvenacava【ABSTRA
3、CT】Objective:Toinvestigatetherenalinjuryofportalhypertensiveratafteronehourofocclusionofportalveinandinferiorvenacava.Methods:HealthymaleWistarratsweretakenrandomlyasnormalcontrol、portalhypertensivecontrolandtrialgroup.Therecoverableportalhypertensivemodelwasinducedfirstly.Th
4、reeweekslater,15modelratsweretakenrandomlyasportalhypertensivecontrolgroup,othershadanotheroperationandweredividedrandomlyinto0,6h,12h,24h,48h,72h,7dgroupaccordingtodifferentreperfusiontimeafter1hourofocclusionofportalveinandinferiorvenacava.Atthecorrespondingtimepointsafterr
5、eperfusion,theexaminationsbelowweredone:serumALT,TBIL,BUN,Crconcentrations;morphologicalchangesofliverandkidney,theultrastructureofrenaltissue.Results:SerumBUNandCrintrailgroupreachedtheirpeakvalue12~24hoursafterreperfusion,thendecreasedgradually,andreturnedtonormal72hoursaft
6、erreperfusion.Themaininjuryofkidneywaslocatedinproximaltubularepithelialcell,itpeakedat12hoursand24hoursafterreperfusion,thesporadickaryopyknosisandkaryorrhexiscouldbeseen,butthebasementmembranepreservedwell.48hourslater,therestorationcouldbeseen.7dayslateritrestoredobviously
7、.Conclusion:Thereareobviousinjuryintubularepithelialcellintheportalhypertensiveratafter1hourofocclusionofportalveinandinferiorvenacava.Buttheinjuryof5kidneyisreversible.【KEYWORDS】Hypertension,portal・Venacava,inferior・Kidney・Rats,Wistar 经典非转流肝移植病人，由于需暂时阻断下
8、腔静脉，肾脏要经历缺血、淤血以及开放后再灌注的过程。本实验旨在通过研究门静脉高压症大鼠阻断门静脉和下腔静脉1h后肾脏损伤的情况，探讨该情
