T淋巴瘤侵袭转移诱导因子1与胃癌细胞侵袭移行能力的关系.doc

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1、T淋巴瘤侵袭转移诱导因子1与胃癌细胞侵袭移行能力的关系作者：朱金明余佩武李翠芳吴淼【摘要】目的检测胃癌细胞中T淋巴瘤侵袭转移诱导因子1(Tlymphomainvasionandmetastasisinducingfactor1，Tiam1)的表达及其与肿瘤侵袭、移行能力的关系，同时观察胃癌细胞在形态学方面的变化。方法采用层黏连蛋白黏附法，由胃癌MKN45细胞株(M0)中筛选获得高(MH)、低(ML)黏附亚株。应用流式细胞仪检测Tiam1在胃癌细胞中的表达，以Boyden小室法测定M0、ML、MH细胞的体外侵袭、移行能

2、力，并分析其与Tiam1表达间的关系，同时采用苏木精伊红及细胞骨架蛋白染色、扫描电镜技术观察胃癌细胞形态。结果MH细胞的体外侵袭、移行能力及Tiam1表达均强于M0、ML细胞(P<0.05)，且Tiam1表达与胃癌细胞体外侵袭、移行能力呈正相关(r=0.997和1.000)；同时与M0、ML相比，MH胞体伸展，表面突起增多，片状伪足宽厚，丝状伪足细长而密集，异型性明显，且细胞骨架结构较紊乱，斑点状肌动蛋白小体增多。但M0、ML细胞间差异无统计学意义(P>0.05)。结论Tiam1表达水平升高可能促进胃癌细

3、胞侵袭、移行。这或许是通过调整胃癌细胞骨架结构重组，增强其变形、游走能力而实现的。【关键词】胃癌；T淋巴瘤侵袭转移诱导因子1；侵袭；移行；形态学　　CorrelationbetweeninvasionandmigrationabilityofgastriccancercellsandTiam1　　【Abstract】ObjectiveToinvestigatetheexpressionofTlymphomainvasionandmetastasisinducingfactor1(Tiam1)ingastriccance

4、rcellsanditsrelationshipwiththeinvasionandmigrationabilityofgastriccancercells,andobservethemorphologicalchangesofgastriccancercells.MethodsHighadhesivesubgroups(MH)andlowadhesivesubgroups(ML)wereseparatedfromMKN45cellstrain(M0)bylamininadhesionmethodinvitro.The

5、expressionsofTiam1inM0,MLandMHcellsweredetectedbyflowcytometry,theinvasionandmigrationabilityofM0,MLandMHcellsinvitrobyBoydenchamber.ThecorrelationbetweeninvasionandmigrationabilityofM0,ML,MHcellsandexpressionofTiam1inM0,ML,MHcellswasanalyzed.Themorphologicaldif

6、ferencesamongM0,MLandMHcellswereobservedbyhematoxylineosinstain,cytoskeletalproteinstainandscanningelectronicmicroscope.ResultsTheinvasionandmigrationabilityandtheexpressionofTiam1inMHcellswerehigherthanthoseinM0andMLcells(P<0.05).TheexpressionofTiam1wasposit

7、ivelycorrelatedwiththeinvasionandmigrationabilityofgastriccancer6cells(r=0.997,1.000).ComparedwiththeM0andMLcells,theMHcellbodieselongatedwithwideandthicklamellatepseudopodia,slenderandintensivefilamentouspseudopodia,moreprojectionsonthesurface,derangementofcell

8、skeletonandmoredotlikeactinbodies.TherewasnosignificantdifferencebetweenM0andMLcells(P>0.05).ConclusionsThehighexpressionlevelofTiam1maypromotetheinvasionandmigra