禾谷缢管蚜谷胱甘肽转移酶分子克隆和功能表达研究

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1、分类号：Q965.9学校代码：10712UDC：595研究生学号:2015071057密级：公开2018届攻读博士学位研究生学位（毕业）论文禾谷缢管蚜谷胱甘肽转移酶分子克隆和功能表达研究学科专业:农业昆虫害虫防治研究方向:昆虫生理生化研究生：BALACHANDARBALAKRISHNAN指导教师:陈茂华教授完成时间:2018年5月中国陕西杨凌Classificationcode:Q965.9Universitycode:10712UDC:595Postgraduatenumber:2015071057Confi

2、dentialitylevel:PublicNorthwestA&FUniversityDissertationforDoctoralDegree,2018IDENTIFICATION,MOLECULARCHARACTERIZATIONANDEXPRESSIONPATTERNOFGLUTATHIONES-TRANSFERASEGENESINTHEBIRDCHERRY-OATAPHIDRHOPALOSIPHUMPADIMajor:AgricultureEntomology&PestControlResearchf

3、ield:InsectPhysiologyandBiochemistryNameofPostgraduate:BalachandarBalakrishnanSupervisor:Prof.MaohuaChenDateofsubmitted:May,2018YanglingShaanxiChinaIDENTIFICATION,MOLECULARCHARACTERIZATIONANDEXPRESSIONPATTERNOFGLUTATHIONES-TRANSFERASEGENESINTHEBIRDCHERRY-OAT

4、APHIDRHOPALOSIPHUMPADIABSTRACTThebirdcherry-oataphid,Rhopalosiphumpadi(L.)isanimportantpestofwheatwhichwidelydistributesintheworld.Itfeedsonsapinthephloemandtransmitsthelotofvirus,includingbarleyyellowdwarfvirus(BYDV).Differentchemicalcompoundsareusedtocontr

5、olthisaphid.R.padihasdevelopedresistancetovariousinsecticides.TheglutathioneS-transferases(GSTs)canprotectinsectagainstoxidativedamagecausedbyROS(reactiveoxygenspecies)andplayroleininsecticideresistanceofaphids.Sixsubclasses(Delta,Epsilon,Omega,Sigma,Thetaan

6、dZeta)ofGTSsarefoundininsects.MostofthepreviousinvestigationshavefocusedondeltaandepsilonGST.However,themolecularmechanismsofGSTsininsecticideresistanceofaphidsstillneedfurtherclarification.Here,wereporttheidentificationandclassificationofGSTgenesfromR.padi.

7、TherecombinantproteinsofsomeGSTgenswereexpressedinEscherichiacolicells.ThebiochemicalpropertiesofthepurifiedrecombinantGSTproteinswerecharacterized.Thetranscriptionalpatternsofsomegenesfollowingexposuretovariousconcentrationsofb-cypermethrin,isoprocarb,malat

8、hion,andsulfoxaflorwereanalyzed.ThepotentialrolesoftheGSTfusionproteinSinantioxidantdefensewerealsoinvestigated.OurresultsprovidevaluableinsightintothefunctionofGSTsinthestressresponsetoinsectic