npy在实验大鼠垂体腺瘤中的表达及意义111

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1、神经肽Y及其受体在实验性大鼠垂体腺瘤中的表达陈来照马景鑑基金项目：国家自然科学基金资助项目（30471772）作者单位：030001太原，山西医科大学第一医院神经外科（陈来照），天津医科大学总医院神经外科（马景鑑）通讯作者：陈来照(E-mail：chenlaizhao@yahoo.com.cn)【摘要】目的研究神经肽Y及其受体在实验大鼠垂体腺瘤中的表达，探索NPY在垂体腺瘤形成过程中的作用。方法雌性Wistar大鼠40只，通过腹腔注射雌激素的方法建立大鼠垂体腺瘤模型，在此基础上采用放射免疫法测定大鼠血浆NPY的浓度，应用免疫组化、免疫电镜和逆转录聚合酶链反应（R

2、T-PCR）的方法分别研究大鼠垂体中NPY及其受体的表达。结果（1）实验组大鼠垂体符合垂体腺瘤的诊断标准；（2）免疫组化和RT-PCR研究表明，在实验组大鼠垂体腺瘤中NPY及其Y1R和Y2R的基因表达水平明显低于对照组垂体。（3）免疫电镜显示NPY阳性颗粒主要位于腺垂体细胞的内分泌颗粒中。结论通过雌激素诱导Wistar大鼠，可以成功建立垂体腺瘤动物模型；实验组大鼠垂体腺瘤中NPY及其受体的表达下调可能与肿瘤的发生有关。【关键词】大鼠；垂体腺瘤；神经肽Y；受体。ExpressionofneuropeptideY(NPY)aswellasitsreceptorsin

3、estrogen-inducedpituitaryadenomainratsCHENLai-zhao﹡,MAJing-jian.﹡DepartmentofNeurosurgery,Firsthospital，ShanXiMedicalUniversity,TanYuan030001,China。【Abstract】ObjectiveTheaimofthisstudywastoexploretheexpressionofneuropeptideYaswellasitsreceptorsinestrogen-inducedpituitarytumorsofWista

4、rrats,toinvestigatetheirrolesinpituitarytumorigenesis.Methods40femaleWistarratsweredividedrandomlyintoexperimentalandcontrolgroups.Theratswereinducedwithestradiolbenzoatebyperitonealinjectioninexperimentalgroup.TheexpressionofNPYaswellasitsreceptorsinpituitaryglandswasalsodetectedbyi

5、mmunohistochemicalanalysisandsemi-quantitativereversetranscriptionpolymerasechainreaction(RT-PCR).Somepituitarytissueswerealsoexaminedusingimmuno-electronmicroscopyinordertostudythedistributionofNPYatthesubcellarlevel.Results(1)Inexperimentalgroup,significantincreasesinpituitaryweigh

6、twerenotedthreemonthsafterperitonealinjectionofestrogen.TheserumprolactinlocatedintheTomb,DongShenJiabang,deferthenextdayfocusedontheassassination.Linping,Zhejiang,1ofwhichliquorwinemasters(WuzhensaidinformationisCarpenter),whogotAfewbayonets,duetomissedfatal,whennightcameconcentrati

7、onalsoincreasedsharply.(2)TheexpressionlevelofNPYinpituitaryofexperimentalgroupsweresignificantlylowercomparedtothatofcontrolgroup(P<0.001).RT-PCRdetectionalsoindicatedthatthegeneexpressionofY1andY2receporsweredecreasedinthepituitarytumors(P<0.001).(3)Immuno-electronmicroscopyexamina

8、tiondemonstr