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1、25-血管内皮祖细胞对共同植入缺血再灌注模型大鼠脑内的神经干细胞增殖、血管内皮祖细胞对共同植入缺血再灌注模型大鼠脑内的神经干细胞增殖、凋亡和血管形成的调节富奇志1，齐志国1,朱晓峰2,谢鹏3(400016重庆，重庆医科大学：第一附属医院神经内科1，神经科学中心3；154002黑龙江佳木斯，佳木斯大学神经科学中心2) ［提要］目的研究血管内皮祖细胞（endothelialprogenitorcells,EPCs）与神经干细胞（neuronstemcells,NSCs）构建的复合移植体对移植入缺血再灌注模型大鼠缺血半暗带的神经干细胞增殖、凋亡和血管重建的影响。方法线栓法建大鼠脑缺血再灌
2、注模型；血管内皮祖细胞、层粘连蛋白和神经干细胞构建的移植复合体移入模型鼠脑缺血半暗带；免疫组化观察Brdu标记的移入脑内神经干细胞和缺血半暗带血管新生，免疫荧光双标观察神经干细胞的凋亡情况。结果移植后各时间点Brdu标记的神经干细胞数NSCs+EPCs组明显高于NSCs组（P<0.05）；NSCs+EPCs组在各时间点的凋亡率明显低于NSCs组（P<0.05）；各时间点NSCs+EPCs组血管新生数量明显多于EPCs组、缺血对照组和NSCs组（P<0.05）。结论血管内皮祖细胞可以促进移植入缺血再灌注模型大鼠缺血半暗带的神经干细胞增殖，减少其凋亡；2种细胞共移植可以促进缺血半暗带的血
3、管新生。［关键词］神经干细胞；毛细血管内皮祖细胞；缺血再灌注 ［中图法分类号］R322.81;R329.28;R743.31［文献标识码］A EndothelialprogenitorcellscombinedimplantationimprovesproliferationandsuppressesapoptosisofneuralstemcellsandangiogenesisofMCAO/RratsFuQizhi1,QiZhiguo1,ZhuXiaofeng2,XiePeng3(1DepartmentofNeurology,FirstAffiliatedHospit
4、al,3NeuroscienceCenter,ChongqingMedicalUniversity,Chongqing,400016;2NeuroscienceCenter,JiamusiUniversity,Jiamusi,HeilongjiangProvince,154002,China)［Abstract］ObjectiveToinvestigatetheeffectofendothelialprogenitorcells(EPCs)whichcomplexedwithneuronstemcells(NSCs)aftertransplantedintoischemiaandre
5、perfusion(I/R)ratmodelontheproliferationandapoptosisoftheNSCsandvasalconstruction.MethodsTotally150SDadultmaleratswererandomlyandequallydividedinto5groups,shamoperationgroup,I/Rgroup,I/R+NSCsgroup,I/R+EPCsgroup,andI/R+NSCs+EPCsgroup.Theratswerefurtherdividedinto5subgroupsaccordingtothetimepoint
6、sof3,7,14,30and60daftertransplantation.I/Rratmodelwasestablishedbyreversiblyligatingmiddlecerebralarteryocclusion.NSCswerederivedfromthehippocampusofSDratsbornin24handidentifiedwithNestinstaining.EPCswereobtainedfromthearterybloodofSDratsandverifiedwithimmunocytochemicalstainingsofCD31,CD34andK
7、DRafterculture.ThecomplexofNSCsandEPCswasproducedwithaidoflaminin.Thenthecomplexweretransplantedintotheischemiapenumbraofcorrespondingmodelratbrain.TheproliferationandapoptosisofNSCs,andthefomationofnewvesselswereobservedthroughim
